Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 2241380..2242180 Replicon chromosome
Accession NZ_CP027457
Organism Escherichia coli strain 88-3493

Toxin (Protein)


Gene name ataT Uniprot ID A0A0K4AFE4
Locus tag C6W79_RS10995 Protein ID WP_001614005.1
Coordinates 2241653..2242180 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag C6W79_RS10990 Protein ID WP_001277108.1
Coordinates 2241380..2241646 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W79_RS10970 2237038..2237706 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
C6W79_RS10975 2237699..2238757 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
C6W79_RS10980 2239002..2239856 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
C6W79_RS10985 2240127..2241230 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
C6W79_RS10990 2241380..2241646 + 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
C6W79_RS10995 2241653..2242180 + 528 WP_001614005.1 GNAT family N-acetyltransferase Toxin
C6W79_RS11000 2242177..2242560 - 384 WP_001614003.1 aspartate 1-decarboxylase autocleavage activator PanM -
C6W79_RS11005 2242984..2244093 + 1110 WP_001301528.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
C6W79_RS11010 2244141..2245067 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
C6W79_RS11015 2245064..2246341 + 1278 WP_001614002.1 branched chain amino acid ABC transporter permease LivM -
C6W79_RS11020 2246338..2247105 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19676.62 Da        Isoelectric Point: 7.3502

>T99002 WP_001614005.1 NZ_CP027457:2241653-2242180 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T99002 NZ_CP027457:2241653-2242180 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT99002 WP_001277108.1 NZ_CP027457:2241380-2241646 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT99002 NZ_CP027457:2241380-2241646 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K4AFE4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References