Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2220042..2220673 Replicon chromosome
Accession NZ_CP027457
Organism Escherichia coli strain 88-3493

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag C6W79_RS10870 Protein ID WP_001259385.1
Coordinates 2220042..2220317 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag C6W79_RS10875 Protein ID WP_000593555.1
Coordinates 2220314..2220673 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W79_RS10855 2215046..2216113 + 1068 WP_001614022.1 HlyD family efflux transporter periplasmic adaptor subunit -
C6W79_RS10860 2216110..2218845 + 2736 WP_001614021.1 ribosome-associated ATPase/putative transporter RbbA -
C6W79_RS10865 2218845..2219969 + 1125 WP_001314210.1 ABC transporter permease -
C6W79_RS10870 2220042..2220317 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
C6W79_RS10875 2220314..2220673 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C6W79_RS10880 2220793..2221194 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
C6W79_RS10885 2221200..2222006 - 807 WP_001614017.1 nickel import ATP-binding protein NikE -
C6W79_RS10890 2222003..2222767 - 765 WP_001614016.1 nickel import ATP-binding protein NikD -
C6W79_RS10895 2222767..2223600 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
C6W79_RS10900 2223597..2224541 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T99001 WP_001259385.1 NZ_CP027457:2220042-2220317 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T99001 NZ_CP027457:2220042-2220317 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT99001 WP_000593555.1 NZ_CP027457:2220314-2220673 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT99001 NZ_CP027457:2220314-2220673 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References