Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 893132..893673 Replicon chromosome
Accession NZ_CP027457
Organism Escherichia coli strain 88-3493

Toxin (Protein)


Gene name yafQ Uniprot ID A0A3W4DAB6
Locus tag C6W79_RS04545 Protein ID WP_024171431.1
Coordinates 893395..893673 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag C6W79_RS04540 Protein ID WP_000729704.1
Coordinates 893132..893392 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W79_RS04520 888844..889629 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
C6W79_RS04525 889601..891313 + 1713 Protein_821 flagellar biosynthesis protein FlhA -
C6W79_RS04530 891500..891997 - 498 WP_001612413.1 REP-associated tyrosine transposase RayT -
C6W79_RS04535 892173..892922 - 750 WP_000056850.1 C40 family peptidase -
C6W79_RS04540 893132..893392 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
C6W79_RS04545 893395..893673 + 279 WP_024171431.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
C6W79_RS04550 893829..894569 + 741 WP_001225679.1 murein L,D-transpeptidase -
C6W79_RS04555 894540..895307 - 768 WP_000333380.1 class II glutamine amidotransferase -
C6W79_RS04560 895513..896091 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10829.68 Da        Isoelectric Point: 10.3349

>T98999 WP_024171431.1 NZ_CP027457:893395-893673 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVKPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T98999 NZ_CP027457:893395-893673 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCAAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTCTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT98999 WP_000729704.1 NZ_CP027457:893132-893392 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT98999 NZ_CP027457:893132-893392 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3W4DAB6

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References