Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 3412226..3412851 Replicon chromosome
Accession NZ_CP027452
Organism Escherichia coli strain 2014C-3338

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag C6W71_RS17505 Protein ID WP_000911329.1
Coordinates 3412453..3412851 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag C6W71_RS17500 Protein ID WP_000450524.1
Coordinates 3412226..3412453 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W71_RS17475 3408026..3408496 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
C6W71_RS17480 3408496..3409068 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
C6W71_RS17485 3409214..3410092 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
C6W71_RS17490 3410109..3411143 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
C6W71_RS17495 3411356..3412069 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
C6W71_RS17500 3412226..3412453 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
C6W71_RS17505 3412453..3412851 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
C6W71_RS17510 3412998..3413861 + 864 WP_042104743.1 neutral zinc metallopeptidase -
C6W71_RS17515 3413876..3415891 + 2016 WP_000829310.1 tRNA cytosine(34) acetyltransferase TmcA -
C6W71_RS17520 3415965..3416663 + 699 WP_000679819.1 esterase -
C6W71_RS17525 3416744..3416944 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T98943 WP_000911329.1 NZ_CP027452:3412453-3412851 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T98943 NZ_CP027452:3412453-3412851 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAACATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTAACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT98943 WP_000450524.1 NZ_CP027452:3412226-3412453 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT98943 NZ_CP027452:3412226-3412453 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References