Detailed information of TA system TA098931

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 1353884..1354591
• Replicon: chromosome
• Accession: NZ_CP027452
• Organism: Escherichia coli strain 2014C-3338

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A4T3Q6D2
• Locus tag: C6W71_RS07065
• Protein ID: WP_001683515.1
• Coordinates: 1353884..1354225 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: C6W71_RS07070
• Protein ID: WP_001296928.1
• Coordinates: 1354256..1354591 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W71_RS07045	1351421..1352218	-	798	WP_032218979.1	helix-turn-helix transcriptional regulator	-
C6W71_RS07050	1352332..1352607	-	276	WP_032218977.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C6W71_RS07055	1352611..1352859	-	249	WP_032218975.1	ribbon-helix-helix domain-containing protein	-
C6W71_RS07060	1352936..1353769	-	834	WP_032218973.1	DUF4942 domain-containing protein	-
C6W71_RS07065	1353884..1354225	-	342	WP_001683515.1	TA system toxin CbtA family protein	Toxin
C6W71_RS07070	1354256..1354591	-	336	WP_001296928.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C6W71_RS07075	1354591..1355064	-	474	WP_001296921.1	DNA repair protein RadC	-
C6W71_RS07080	1355094..1355912	-	819	WP_024223204.1	DUF945 domain-containing protein	-
C6W71_RS07085	1356149..1357102	-	954	WP_052920587.1	hypothetical protein	-
C6W71_RS07090	1357695..1358309	+	615	WP_000772910.1	inovirus Gp2 family protein	-
C6W71_RS07095	1358427..1358645	+	219	WP_042106713.1	AlpA family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB	1341329..1371371	30042

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-110)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13034.05 Da
• Isoelectric Point: 9.5454

>T98931 WP_001683515.1 NZ_CP027452:c1354225-1353884 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVDAVNFLVEKYELIRIDRKGF
SWQEQTPYLRAVDILRARQATGLLRRCHNTTAR

Nucleotide

• Length: 342 bp

>T98931 NZ_CP027452:c1354225-1353884 [Escherichia coli]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCGCCCGTCGTGGTCTGGCAAACATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGACTGAACCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCGATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGATCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTACGGGCGCGACAGGCTACAGGTCTGCTGCGCCGCTG
CCATAACACCACGGCACGGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12565.57 Da
• Isoelectric Point: 7.7245

>AT98931 WP_001296928.1 NZ_CP027452:c1354591-1354256 [Escherichia coli]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKHLELKLADGELSPCQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT98931 NZ_CP027452:c1354591-1354256 [Escherichia coli]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGCTGATGAAACATCTGGAGCTGAAACTGGCTGACGGTGAACTCTCTCCCTGCCAACAACGT
ACCGTCACGCTGTACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Structures

Toxin

• AlphaFold DB: A0A4T3Q6D2