Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 76256..76781 Replicon plasmid unnamed2
Accession NZ_CP027451
Organism Escherichia coli strain 2014C-3097

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag C6W73_RS27110 Protein ID WP_001159871.1
Coordinates 76476..76781 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag C6W73_RS27105 Protein ID WP_000813639.1
Coordinates 76256..76474 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W73_RS28155 72200..72417 + 218 Protein_65 hypothetical protein -
C6W73_RS27065 72421..72531 + 111 Protein_66 IS481 family transposase -
C6W73_RS27075 72803..73534 + 732 WP_000850414.1 complement resistance protein TraT -
C6W73_RS27080 73594..73965 + 372 WP_001373114.1 colicin -
C6W73_RS27090 74448..74795 + 348 WP_000142427.1 hypothetical protein -
C6W73_RS27095 74813..75403 - 591 WP_033802802.1 hypothetical protein -
C6W73_RS27100 75400..75660 - 261 WP_000343100.1 hypothetical protein -
C6W73_RS27105 76256..76474 + 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C6W73_RS27110 76476..76781 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
C6W73_RS27115 76782..77588 + 807 WP_000016966.1 site-specific integrase -
C6W73_RS27120 77768..78412 + 645 WP_001144036.1 ParA family protein -
C6W73_RS27125 78499..78807 + 309 WP_000030199.1 hypothetical protein -
C6W73_RS27130 79222..80202 + 981 WP_000688510.1 plasmid segregation protein ParM -
C6W73_RS27135 80195..80611 + 417 WP_001278809.1 plasmid partitioning/stability family protein -
C6W73_RS27140 80613..80786 - 174 Protein_79 DUF4113 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - espP / hlyC / hlyA / hlyB / hlyD 1..173649 173649


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T98923 WP_001159871.1 NZ_CP027451:76476-76781 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T98923 NZ_CP027451:76476-76781 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT98923 WP_000813639.1 NZ_CP027451:76256-76474 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT98923 NZ_CP027451:76256-76474 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGCCTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References