Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2422942..2423573 Replicon chromosome
Accession NZ_CP027449
Organism Escherichia coli strain 2014C-3097

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag C6W73_RS12350 Protein ID WP_001259385.1
Coordinates 2422942..2423217 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q31VE4
Locus tag C6W73_RS12355 Protein ID WP_000593554.1
Coordinates 2423214..2423573 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W73_RS12335 2417946..2419013 + 1068 WP_000361467.1 HlyD family secretion protein -
C6W73_RS12340 2419010..2421745 + 2736 WP_000149165.1 ribosome-associated ATPase/putative transporter RbbA -
C6W73_RS12345 2421745..2422869 + 1125 WP_001314210.1 ABC transporter permease -
C6W73_RS12350 2422942..2423217 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
C6W73_RS12355 2423214..2423573 + 360 WP_000593554.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C6W73_RS12360 2423623..2424483 - 861 WP_001131783.1 class II aldolase -
C6W73_RS12365 2424515..2424784 - 270 WP_000933060.1 HPr family phosphocarrier protein -
C6W73_RS12370 2424774..2426282 - 1509 WP_001298718.1 carbohydrate kinase -
C6W73_RS12375 2426275..2427633 - 1359 WP_001298729.1 PTS galactitol transporter subunit IIC -
C6W73_RS12380 2427710..2427991 - 282 WP_000084022.1 PTS sugar transporter subunit IIB -
C6W73_RS12385 2427988..2428461 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2415325..2429832 14507


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T98905 WP_001259385.1 NZ_CP027449:2422942-2423217 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T98905 NZ_CP027449:2422942-2423217 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13359.25 Da        Isoelectric Point: 4.8838

>AT98905 WP_000593554.1 NZ_CP027449:2423214-2423573 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT98905 NZ_CP027449:2423214-2423573 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCTAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A6H2GM94

References