Detailed information of TA system TA098830

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4140197..4140609
• Replicon: chromosome
• Accession: NZ_CP027442
• Organism: Escherichia coli strain 2013C-3252

Toxin (Protein)

• Gene name: symE
• Uniprot ID: A0A0F3WCK2
• Locus tag: MJ69_RS22745
• Protein ID: WP_000132621.1
• Coordinates: 4140197..4140538 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4140533..4140609 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ69_RS22730	4136207..4137619	-	1413	WP_000199352.1	PLP-dependent aminotransferase family protein	-
MJ69_RS22735	4137796..4137960	+	165	WP_000394276.1	DUF1127 domain-containing protein	-
MJ69_RS22740	4138057..4140150	+	2094	WP_000648228.1	DUF262 domain-containing protein	-
MJ69_RS22745	4140197..4140538	-	342	WP_000132621.1	endoribonuclease SymE	Toxin
-	4140533..4140609	+	77	NuclAT_27	-	Antitoxin
-	4140533..4140609	+	77	NuclAT_27	-	Antitoxin
-	4140533..4140609	+	77	NuclAT_27	-	Antitoxin
-	4140533..4140609	+	77	NuclAT_27	-	Antitoxin
-	4140533..4140609	+	77	NuclAT_28	-	Antitoxin
-	4140533..4140609	+	77	NuclAT_28	-	Antitoxin
-	4140533..4140609	+	77	NuclAT_28	-	Antitoxin
-	4140533..4140609	+	77	NuclAT_28	-	Antitoxin
MJ69_RS22750	4140759..4142528	-	1770	WP_000110079.1	restriction endonuclease subunit S	-
MJ69_RS22755	4142528..4143997	-	1470	WP_106907404.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12342.12 Da
• Isoelectric Point: 7.8218

>T98830 WP_000132621.1 NZ_CP027442:c4140538-4140197 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPTAEESE
LMQSLRQVCKLSARKQKQVQDFIGVITGKQKVA

Nucleotide

• Length: 342 bp

>T98830 NZ_CP027442:c4140538-4140197 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCACCGCCGAAGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAAGCAGGTGCAGGATTTTATTGGGGTAATTAC
CGGTAAACAGAAAGTCGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT98830 NZ_CP027442:4140533-4140609 [Escherichia coli]
AGTCATAACTGCTATTCTCCGATAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0F3WCK2

Antitoxin