Detailed information of TA system TA098754

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 662510..663148
• Replicon: chromosome
• Accession: NZ_CP027437
• Organism: Escherichia coli strain 2012C-4221

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B7N4J4
• Locus tag: C6W65_RS03560
• Protein ID: WP_000813797.1
• Coordinates: 662972..663148 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C6W65_RS03555
• Protein ID: WP_076611057.1
• Coordinates: 662510..662926 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W65_RS03535	657662..658603	-	942	WP_001251315.1	ABC transporter permease	-
C6W65_RS03540	658604..659617	-	1014	WP_085453819.1	ABC transporter ATP-binding protein	-
C6W65_RS03545	659635..660780	-	1146	WP_106905996.1	ABC transporter substrate-binding protein	-
C6W65_RS03550	661025..662431	-	1407	WP_000760596.1	PLP-dependent aminotransferase family protein	-
C6W65_RS03555	662510..662926	-	417	WP_076611057.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
C6W65_RS03560	662972..663148	-	177	WP_000813797.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
C6W65_RS03565	663370..663600	+	231	WP_000494244.1	YncJ family protein	-
C6W65_RS03570	663692..665653	-	1962	WP_024216495.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
C6W65_RS03575	665726..666262	-	537	WP_106905997.1	DNA-binding transcriptional regulator SutR	-
C6W65_RS03580	666315..667526	+	1212	WP_106905998.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.76 Da
• Isoelectric Point: 11.5666

>T98754 WP_000813797.1 NZ_CP027437:c663148-662972 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T98754 NZ_CP027437:c663148-662972 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15251.65 Da
• Isoelectric Point: 4.5908

>AT98754 WP_076611057.1 NZ_CP027437:c662926-662510 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT98754 NZ_CP027437:c662926-662510 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A829J715