Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 35048..35573 Replicon plasmid unnamed2
Accession NZ_CP027370
Organism Escherichia coli strain 2014C-3307

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag C6P71_RS26770 Protein ID WP_001159871.1
Coordinates 35048..35353 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag C6P71_RS26775 Protein ID WP_000813639.1
Coordinates 35355..35573 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6P71_RS26740 31042..31215 + 174 Protein_42 DUF4113 domain-containing protein -
C6P71_RS26745 31217..31633 - 417 WP_001278818.1 plasmid partitioning/stability family protein -
C6P71_RS26750 31626..32606 - 981 WP_096955086.1 plasmid segregation protein ParM -
C6P71_RS26755 33021..33329 - 309 WP_000030199.1 hypothetical protein -
C6P71_RS26760 33416..34060 - 645 WP_001144036.1 ParA family protein -
C6P71_RS26765 34241..35047 - 807 WP_000016966.1 site-specific integrase -
C6P71_RS26770 35048..35353 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
C6P71_RS26775 35355..35573 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C6P71_RS26780 36169..36429 + 261 WP_047086508.1 hypothetical protein -
C6P71_RS26785 36426..37016 + 591 WP_000194569.1 hypothetical protein -
C6P71_RS26790 37034..37381 - 348 WP_000142427.1 hypothetical protein -
C6P71_RS26800 37864..38235 - 372 WP_001373114.1 colicin -
C6P71_RS26805 38295..39026 - 732 WP_000850414.1 complement resistance protein TraT -
C6P71_RS26815 39298..39408 - 111 Protein_55 IS481 family transposase -
C6P71_RS28000 39412..39629 - 218 Protein_56 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - hlyD / hlyB / hlyA / hlyC / espP / espP 1..176149 176149


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T98400 WP_001159871.1 NZ_CP027370:c35353-35048 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T98400 NZ_CP027370:c35353-35048 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCTTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT98400 WP_000813639.1 NZ_CP027370:c35573-35355 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT98400 NZ_CP027370:c35573-35355 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGCCTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References