Detailed information of TA system TA098322

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5289020..5289645
• Replicon: chromosome
• Accession: NZ_CP027361
• Organism: Escherichia coli strain 2014C-4639

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: RR62_RS26925
• Protein ID: WP_000911330.1
• Coordinates: 5289020..5289418 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: RR62_RS26930
• Protein ID: WP_000450524.1
• Coordinates: 5289418..5289645 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RR62_RS26905 (5284898)	5284898..5285098	+	201	WP_000383836.1	YpfN family protein	-
RR62_RS26910 (5285208)	5285208..5285906	-	699	WP_000679812.1	esterase	-
RR62_RS26915 (5285980)	5285980..5287995	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
RR62_RS26920 (5288010)	5288010..5288873	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
RR62_RS26925 (5289020)	5289020..5289418	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
RR62_RS26930 (5289418)	5289418..5289645	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
RR62_RS26935 (5289799)	5289799..5290512	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
RR62_RS26940 (5290725)	5290725..5291759	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
RR62_RS26945 (5291776)	5291776..5292654	-	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
RR62_RS26950 (5292800)	5292800..5293372	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
RR62_RS26955 (5293372)	5293372..5293842	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T98322 WP_000911330.1 NZ_CP027361:c5289418-5289020 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T98322 NZ_CP027361:c5289418-5289020 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT98322 WP_000450524.1 NZ_CP027361:c5289645-5289418 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT98322 NZ_CP027361:c5289645-5289418 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33