Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2719644..2720298 Replicon chromosome
Accession NZ_CP027352
Organism Escherichia coli strain 2012C-4606

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag AYN42_RS15335 Protein ID WP_000244777.1
Coordinates 2719644..2720051 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AYN42_RS15340 Protein ID WP_000354046.1
Coordinates 2720032..2720298 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYN42_RS15315 2715601..2717334 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
AYN42_RS15320 2717340..2718050 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AYN42_RS15325 2718075..2718971 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AYN42_RS15330 2719083..2719604 + 522 WP_001055874.1 flavodoxin FldB -
AYN42_RS15335 2719644..2720051 - 408 WP_000244777.1 toxin CptA Toxin
AYN42_RS15340 2720032..2720298 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AYN42_RS15345 2720541..2721521 + 981 WP_000886095.1 tRNA-modifying protein YgfZ -
AYN42_RS15350 2721717..2722376 - 660 WP_000250274.1 hemolysin III family protein -
AYN42_RS15355 2722540..2722851 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
AYN42_RS15360 2722896..2724329 + 1434 WP_000737106.1 6-phospho-beta-glucosidase BglA -
AYN42_RS15365 2724386..2725129 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T98237 WP_000244777.1 NZ_CP027352:c2720051-2719644 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T98237 NZ_CP027352:c2720051-2719644 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT98237 WP_000354046.1 NZ_CP027352:c2720298-2720032 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT98237 NZ_CP027352:c2720298-2720032 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References