Detailed information of TA system TA098042

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3695683..3696285
• Replicon: chromosome
• Accession: NZ_CP027331
• Organism: Escherichia coli strain 2013C-3277

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: MJ63_RS20155
• Protein ID: WP_000897305.1
• Coordinates: 3695974..3696285 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MJ63_RS20150
• Protein ID: WP_000356397.1
• Coordinates: 3695683..3695973 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ63_RS20120	3691607..3692509	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
MJ63_RS20125	3692506..3693141	+	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
MJ63_RS20130	3693138..3694067	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
MJ63_RS20135	3694398..3694640	-	243	WP_001086388.1	hypothetical protein	-
MJ63_RS20140	3694859..3695077	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
MJ63_RS20150	3695683..3695973	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
MJ63_RS20155	3695974..3696285	-	312	WP_000897305.1	hypothetical protein	Toxin
MJ63_RS20160	3696514..3697422	+	909	WP_001162704.1	alpha/beta hydrolase	-
MJ63_RS20165	3697486..3698427	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
MJ63_RS20170	3698472..3698909	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
MJ63_RS20175	3698906..3699778	-	873	WP_000920762.1	virulence factor BrkB family protein	-
MJ63_RS20180	3699772..3700371	-	600	WP_001315111.1	glucose-1-phosphatase	-
MJ63_RS20185	3700470..3701255	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T98042 WP_000897305.1 NZ_CP027331:c3696285-3695974 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T98042 NZ_CP027331:c3696285-3695974 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT98042 WP_000356397.1 NZ_CP027331:c3695973-3695683 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT98042 NZ_CP027331:c3695973-3695683 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7