Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1810095..1810597 Replicon chromosome
Accession NZ_CP027331
Organism Escherichia coli strain 2013C-3277

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag MJ63_RS10195 Protein ID WP_000767829.1
Coordinates 1810095..1810349 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag MJ63_RS10200 Protein ID WP_001259255.1
Coordinates 1810346..1810597 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ63_RS10165 1805110..1805337 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
MJ63_RS10170 1805351..1806409 - 1059 WP_000492324.1 transport protein YeeE -
MJ63_RS10175 1806588..1807946 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
MJ63_RS10180 1807936..1807998 - 63 WP_010723108.1 membrane protein YoeI -
MJ63_RS10185 1808213..1809142 - 930 WP_000803351.1 LysR family transcriptional regulator -
MJ63_RS10190 1809188..1810012 - 825 WP_000754737.1 SDR family oxidoreductase -
MJ63_RS10195 1810095..1810349 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
MJ63_RS10200 1810346..1810597 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
MJ63_RS10205 1810880..1810927 + 48 WP_001375657.1 his operon leader peptide -
MJ63_RS10215 1811073..1811972 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
MJ63_RS10220 1811978..1813282 + 1305 WP_000009589.1 histidinol dehydrogenase -
MJ63_RS10225 1813279..1814349 + 1071 WP_000108914.1 histidinol-phosphate transaminase -
MJ63_RS10230 1814349..1815416 + 1068 WP_000080089.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T98036 WP_000767829.1 NZ_CP027331:c1810349-1810095 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T98036 NZ_CP027331:c1810349-1810095 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT98036 WP_001259255.1 NZ_CP027331:c1810597-1810346 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT98036 NZ_CP027331:c1810597-1810346 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References