Detailed information of TA system TA098035

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1796832..1797667
• Replicon: chromosome
• Accession: NZ_CP027331
• Organism: Escherichia coli strain 2013C-3277

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A136X5A2
• Locus tag: MJ63_RS10110
• Protein ID: WP_000854757.1
• Coordinates: 1797290..1797667 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: MJ63_RS10105
• Protein ID: WP_001295723.1
• Coordinates: 1796832..1797200 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ63_RS10075	1792120..1792500	+	381	Protein_1839	DUF932 domain-containing protein	-
MJ63_RS10080	1793010..1794788	+	1779	WP_001243195.1	reverse transcriptase/maturase family protein	-
MJ63_RS10085	1794907..1795347	+	441	Protein_1841	DUF932 domain-containing protein	-
MJ63_RS10090	1795402..1795887	+	486	WP_000849582.1	antirestriction protein	-
MJ63_RS10095	1795903..1796379	+	477	WP_001186725.1	RadC family protein	-
MJ63_RS10100	1796448..1796669	+	222	WP_000692323.1	DUF987 domain-containing protein	-
MJ63_RS10105	1796832..1797200	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MJ63_RS10110	1797290..1797667	+	378	WP_000854757.1	TA system toxin CbtA family protein	Toxin
MJ63_RS10115	1797664..1797867	+	204	WP_000761705.1	hypothetical protein	-
MJ63_RS31190	1797880..1797993	+	114	WP_001161660.1	DUF957 domain-containing protein	-
MJ63_RS10125	1798152..1799180	+	1029	Protein_1849	IS110 family transposase	-
MJ63_RS10135	1799634..1799816	+	183	WP_024167529.1	ethanolamine utilization protein	-
MJ63_RS10140	1799917..1800246	-	330	WP_000450409.1	DUF496 family protein	-
MJ63_RS10145	1800418..1801476	-	1059	WP_001200887.1	FUSC family protein	-
MJ63_RS10150	1801674..1802147	-	474	WP_001105384.1	DNA gyrase inhibitor SbmC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14153.18 Da
• Isoelectric Point: 7.3249

>T98035 WP_000854757.1 NZ_CP027331:1797290-1797667 [Escherichia coli]
MKTLPDTHVREVSCCPSLVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T98035 NZ_CP027331:1797290-1797667 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCTCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAACAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT98035 WP_001295723.1 NZ_CP027331:1796832-1797200 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT98035 NZ_CP027331:1796832-1797200 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A136X5A2

Antitoxin

• AlphaFold DB: S1NM52