Detailed information of TA system TA098009

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 66607..67166
• Replicon: plasmid unnamed1
• Accession: NZ_CP027326
• Organism: Escherichia coli strain 2013C-4830

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C6N65_RS27745
• Protein ID: WP_032206862.1
• Coordinates: 66891..67166 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: C6N65_RS27740
• Protein ID: WP_001178089.1
• Coordinates: 66607..66891 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N65_RS27695	62243..62455	+	213	WP_001302189.1	ANR family transcriptional regulator	-
C6N65_RS27700	62700..63161	+	462	WP_047619094.1	thermonuclease family protein	-
C6N65_RS27705	63207..63416	+	210	WP_047619093.1	gene expression modulator	-
C6N65_RS27710	63454..64044	+	591	WP_000766812.1	DUF2726 domain-containing protein	-
C6N65_RS27715	64284..64538	+	255	WP_000083830.1	replication regulatory protein RepA	-
C6N65_RS27725	64774..64848	+	75	WP_001370046.1	RepA leader peptide Tap	-
C6N65_RS27730	64841..65698	+	858	WP_032206860.1	incFII family plasmid replication initiator RepA	-
C6N65_RS27740	66607..66891	+	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
C6N65_RS27745	66891..67166	+	276	WP_032206862.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6N65_RS27750	67261..67467	+	207	WP_001105064.1	hypothetical protein	-
C6N65_RS27755	67647..68824	-	1178	Protein_71	IS91 family transposase	-
C6N65_RS27760	68824..69189	-	366	WP_000091308.1	hypothetical protein	-
C6N65_RS27765	69226..70370	+	1145	WP_167588606.1	IS3 family transposase	-
C6N65_RS27770	70411..70782	+	372	Protein_74	chromosome partitioning protein ParB	-
C6N65_RS27775	70921..71094	-	174	Protein_75	DUF4113 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hlyD / hlyB / hlyA / hlyC / papC / espP	1..74671	74671

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10680.34 Da
• Isoelectric Point: 9.5302

>T98009 WP_032206862.1 NZ_CP027326:66891-67166 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENS

Nucleotide

• Length: 276 bp

>T98009 NZ_CP027326:66891-67166 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGTTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT98009 WP_001178089.1 NZ_CP027326:66607-66891 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT98009 NZ_CP027326:66607-66891 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCTTGGACGATGTGACATCCGGACAGGTTATTGACCATCAAGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A731IHA8