Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 5331396..5331937 Replicon chromosome
Accession NZ_CP027323
Organism Escherichia coli strain 2013C-3033

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag C6N24_RS28160 Protein ID WP_000615982.1
Coordinates 5331396..5331674 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag C6N24_RS28165 Protein ID WP_000729704.1
Coordinates 5331677..5331937 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N24_RS28145 5328978..5329556 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
C6N24_RS28150 5329762..5330529 + 768 WP_000333380.1 class II glutamine amidotransferase -
C6N24_RS28155 5330500..5331240 - 741 WP_001225679.1 murein L,D-transpeptidase -
C6N24_RS28160 5331396..5331674 - 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
C6N24_RS28165 5331677..5331937 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
C6N24_RS28170 5332123..5332896 + 774 WP_000543899.1 C40 family peptidase -
C6N24_RS28175 5332953..5333309 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
C6N24_RS28180 5333302..5333580 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
C6N24_RS28185 5333685..5335397 - 1713 Protein_5156 flagellar biosynthesis protein FlhA -
C6N24_RS28190 5335369..5336154 + 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T97979 WP_000615982.1 NZ_CP027323:c5331674-5331396 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T97979 NZ_CP027323:c5331674-5331396 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT97979 WP_000729704.1 NZ_CP027323:c5331937-5331677 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT97979 NZ_CP027323:c5331937-5331677 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References