Detailed information of TA system TA097844

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2729382..2730007
• Replicon: chromosome
• Accession: NZ_CP027310
• Organism: Escherichia coli strain 2014C-4135

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C6N22_RS14025
• Protein ID: WP_000911330.1
• Coordinates: 2729609..2730007 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: C6N22_RS14020
• Protein ID: WP_000450524.1
• Coordinates: 2729382..2729609 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N22_RS13995	2725186..2725656	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
C6N22_RS14000	2725656..2726228	-	573	WP_000176191.1	glycine cleavage system transcriptional repressor	-
C6N22_RS14005	2726374..2727252	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
C6N22_RS14010	2727269..2728303	+	1035	WP_001358397.1	outer membrane protein assembly factor BamC	-
C6N22_RS14015	2728516..2729229	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
C6N22_RS14020	2729382..2729609	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
C6N22_RS14025	2729609..2730007	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C6N22_RS14030	2730154..2731017	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
C6N22_RS14035	2731032..2733047	+	2016	WP_044862802.1	tRNA cytosine(34) acetyltransferase TmcA	-
C6N22_RS14040	2733121..2733818	+	698	Protein_2529	esterase	-
C6N22_RS14045	2733928..2734128	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T97844 WP_000911330.1 NZ_CP027310:2729609-2730007 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T97844 NZ_CP027310:2729609-2730007 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT97844 WP_000450524.1 NZ_CP027310:2729382-2729609 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT97844 NZ_CP027310:2729382-2729609 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33