Detailed information of TA system TA097827

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 41675..42101
• Replicon: plasmid unnamed1
• Accession: NZ_CP027308
• Organism: Escherichia coli strain 2015C-3108

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: C6N27_RS28925
• Protein ID: WP_001312861.1
• Coordinates: 41675..41833 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	41877..42101 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N27_RS28885	37045..37734	-	690	WP_000283394.1	conjugal transfer transcriptional regulator TraJ	-
C6N27_RS28890	37921..38304	-	384	WP_001151524.1	relaxosome protein TraM	-
C6N27_RS28895	38718..39227	+	510	Protein_36	transglycosylase SLT domain-containing protein	-
C6N27_RS28900	39524..40345	-	822	WP_001234469.1	DUF945 domain-containing protein	-
C6N27_RS28905	40466..40753	-	288	WP_000107535.1	hypothetical protein	-
C6N27_RS30410	41097..41321	+	225	WP_001427866.1	hypothetical protein	-
C6N27_RS28925	41675..41833	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	41877..42101	-	225	NuclAT_0	-	Antitoxin
-	41877..42101	-	225	NuclAT_0	-	Antitoxin
-	41877..42101	-	225	NuclAT_0	-	Antitoxin
-	41877..42101	-	225	NuclAT_0	-	Antitoxin
C6N27_RS30415	41913..42101	+	189	WP_001299721.1	hypothetical protein	-
C6N27_RS28930	42070..42833	-	764	Protein_42	plasmid SOS inhibition protein A	-
C6N27_RS28935	42830..43264	-	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
C6N27_RS28940	43319..45277	-	1959	WP_001145472.1	ParB/RepB/Spo0J family partition protein	-
C6N27_RS28945	45336..45569	-	234	WP_000006004.1	DUF905 domain-containing protein	-
C6N27_RS28950	45625..46152	-	528	WP_000290792.1	single-stranded DNA-binding protein	-
C6N27_RS28970	46625..46891	-	267	WP_071828223.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyA / hlyA / hlyA / hlyC	1..63664	63664

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T97827 WP_001312861.1 NZ_CP027308:c41833-41675 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T97827 NZ_CP027308:c41833-41675 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT97827 NZ_CP027308:c42101-41877 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin