Detailed information of TA system TA097591

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 33717..34368
• Replicon: plasmid unnamed4
• Accession: NZ_CP027186
• Organism: Acinetobacter baumannii strain AR_0052

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: CSB68_RS21195
• Protein ID: WP_000269904.1
• Coordinates: 33717..34073 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CSB68_RS21200
• Protein ID: WP_001129974.1
• Coordinates: 34066..34368 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSB68_RS21175	29856..31023	-	1168	Protein_47	MobA/MobL family protein	-
CSB68_RS21180	31327..32087	+	761	Protein_48	hypothetical protein	-
CSB68_RS21185	32234..32664	-	431	Protein_49	MarR family transcriptional regulator	-
CSB68_RS21190	32671..33099	-	429	WP_000105988.1	organic hydroperoxide resistance protein	-
CSB68_RS21195	33717..34073	+	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSB68_RS21200	34066..34368	+	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
CSB68_RS21205	34361..34570	+	210	WP_000069474.1	hypothetical protein	-
CSB68_RS21210	34904..35119	+	216	WP_000071892.1	cold-shock protein	-
CSB68_RS21215	35217..35444	+	228	WP_000921865.1	hypothetical protein	-
CSB68_RS21220	35511..35708	+	198	WP_000476222.1	hypothetical protein	-
CSB68_RS21225	35895..36215	+	321	WP_000897309.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CSB68_RS21230	36208..36480	+	273	WP_000369782.1	helix-turn-helix domain-containing protein	-
CSB68_RS21235	37052..37480	+	429	WP_000105988.1	organic hydroperoxide resistance protein	-
CSB68_RS21240	37487..37918	+	432	WP_000042163.1	MarR family transcriptional regulator	-
CSB68_RS21245	38158..38505	-	348	Protein_61	plasmid replication DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..38505	38505

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T97591 WP_000269904.1 NZ_CP027186:33717-34073 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T97591 NZ_CP027186:33717-34073 [Acinetobacter baumannii]
ATGTGGACAGTCATTACAACGGATCTGTTTAATGAATGGCTTGAACAGCAAGATGAAGCAACACAAGAGAAAGTCTTAGC
TGCATTGGTTGTTCTACAACAGCAAGGGCCTAGTTTAGGTCGTCCTTTAGTAGATACCGTCTACGATTCTAAATTTACCA
ATATGAAGGAACTACGTGTTCAGCATCGTGGTAAACCGTTAAGGGCATTTTTTGCATTCGATCCCCTAAGACAAGCGATT
GTGCTGTGTATTGGTGATAAGAGTAATAAAAAGCGTTTTTATACAGAGATGCTAGCAATTGCAGATGAGCAATACGCACT
TCATCTCTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT97591 WP_001129974.1 NZ_CP027186:34066-34368 [Acinetobacter baumannii]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT97591 NZ_CP027186:34066-34368 [Acinetobacter baumannii]
ATGGCTAAGAGCTTACAAGAATTATTGGCTAGTCGCTCTCCAGAGAGTCAAGCTCGTATTCAAAAAATGGCTGATGAATT
ACTTTTAGAAAGTCAGCTTCACCTTATCCGAGAAGAGTTAGAAATTTCCCAGAAAGAACTAGCTGCAACCCTTGGAATTA
AACAGCCGTCATTATCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTTGAAGCAATG
GGTGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGGTTCAATGTCTAA

Structures

Toxin

• AlphaFold DB: N8YFZ1