Detailed information of TA system TA097590

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 25352..26003
• Replicon: plasmid unnamed4
• Accession: NZ_CP027186
• Organism: Acinetobacter baumannii strain AR_0052

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: CSB68_RS21150
• Protein ID: WP_000269904.1
• Coordinates: 25647..26003 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CSB68_RS21145
• Protein ID: WP_001129974.1
• Coordinates: 25352..25654 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSB68_RS21100	21044..21562	+	519	WP_000839337.1	plasmid replication DNA-binding protein	-
CSB68_RS21105	21802..22233	-	432	WP_000042163.1	MarR family transcriptional regulator	-
CSB68_RS21110	22240..22668	-	429	WP_000105988.1	organic hydroperoxide resistance protein	-
CSB68_RS21115	23240..23512	-	273	WP_000369782.1	helix-turn-helix domain-containing protein	-
CSB68_RS21120	23505..23825	-	321	WP_000897309.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CSB68_RS21125	24012..24209	-	198	WP_000476222.1	hypothetical protein	-
CSB68_RS21130	24276..24503	-	228	WP_000921865.1	hypothetical protein	-
CSB68_RS21135	24601..24816	-	216	WP_000071892.1	cold-shock protein	-
CSB68_RS21140	25150..25359	-	210	WP_000069474.1	hypothetical protein	-
CSB68_RS21145	25352..25654	-	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
CSB68_RS21150	25647..26003	-	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSB68_RS21155	26622..27050	+	429	WP_000105988.1	organic hydroperoxide resistance protein	-
CSB68_RS21160	27057..27488	+	432	WP_000042163.1	MarR family transcriptional regulator	-
CSB68_RS21165	27728..28246	-	519	WP_000839337.1	plasmid replication DNA-binding protein	-
CSB68_RS21170	28324..29259	-	936	WP_001292329.1	replication initiation protein RepM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..38505	38505

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T97590 WP_000269904.1 NZ_CP027186:c26003-25647 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T97590 NZ_CP027186:c26003-25647 [Acinetobacter baumannii]
ATGTGGACAGTCATTACAACGGATCTGTTTAATGAATGGCTTGAACAGCAAGATGAAGCAACACAAGAGAAAGTCTTAGC
TGCATTGGTTGTTCTACAACAGCAAGGGCCTAGTTTAGGTCGTCCTTTAGTAGATACCGTCTACGATTCTAAATTTACCA
ATATGAAGGAACTACGTGTTCAGCATCGTGGTAAACCGTTAAGGGCATTTTTTGCATTCGATCCCCTAAGACAAGCGATT
GTGCTGTGTATTGGTGATAAGAGTAATAAAAAGCGTTTTTATACAGAGATGCTAGCAATTGCAGATGAGCAATACGCACT
TCATCTCTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT97590 WP_001129974.1 NZ_CP027186:c25654-25352 [Acinetobacter baumannii]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT97590 NZ_CP027186:c25654-25352 [Acinetobacter baumannii]
ATGGCTAAGAGCTTACAAGAATTATTGGCTAGTCGCTCTCCAGAGAGTCAAGCTCGTATTCAAAAAATGGCTGATGAATT
ACTTTTAGAAAGTCAGCTTCACCTTATCCGAGAAGAGTTAGAAATTTCCCAGAAAGAACTAGCTGCAACCCTTGGAATTA
AACAGCCGTCATTATCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTTGAAGCAATG
GGTGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGGTTCAATGTCTAA

Structures

Toxin

• AlphaFold DB: N8YFZ1