Detailed information of TA system TA097435

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 36577..36841
• Replicon: plasmid unnamed3
• Accession: NZ_CP027135
• Organism: Escherichia coli strain AR_0369

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: CSC06_RS00205
• Protein ID: WP_001331364.1
• Coordinates: 36577..36729 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	36779..36841 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC06_RS00180	31852..34020	+	2169	WP_000698357.1	DotA/TraY family protein	-
CSC06_RS00185	34094..34744	+	651	WP_001178506.1	plasmid IncI1-type surface exclusion protein ExcA	-
CSC06_RS00190	34816..35025	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
CSC06_RS26335	35417..35593	+	177	WP_001054904.1	hypothetical protein	-
CSC06_RS00195	35658..35954	-	297	WP_001275298.1	hypothetical protein	-
CSC06_RS00200	36254..36505	+	252	WP_001291964.1	hypothetical protein	-
CSC06_RS00205	36577..36729	-	153	WP_001331364.1	Hok/Gef family protein	Toxin
-	36779..36841	+	63	NuclAT_0	-	Antitoxin
-	36779..36841	+	63	NuclAT_0	-	Antitoxin
-	36779..36841	+	63	NuclAT_0	-	Antitoxin
-	36779..36841	+	63	NuclAT_0	-	Antitoxin
CSC06_RS00210	37021..38229	+	1209	WP_001383960.1	IncI1-type conjugal transfer protein TrbA	-
CSC06_RS00215	38248..39318	+	1071	WP_105394063.1	IncI1-type conjugal transfer protein TrbB	-
CSC06_RS00220	39311..41602	+	2292	WP_105394064.1	conjugal transfer protein TrbC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaCTX-M-15 / blaTEM-1D	iutA / iucD / iucC / iucB / iucA	1..151026	151026

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T97435 WP_001331364.1 NZ_CP027135:c36729-36577 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T97435 NZ_CP027135:c36729-36577 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT97435 NZ_CP027135:36779-36841 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin