Detailed information of TA system TA097288

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 65721..65991
• Replicon: plasmid pRM14721
• Accession: NZ_CP027106
• Organism: Escherichia coli strain RM14721

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: C5097_RS24715
• Protein ID: WP_001312861.1
• Coordinates: 65833..65991 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	65721..65784 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5097_RS24685	61021..61818	+	798	WP_123001400.1	IS21-like element IS21 family helper ATPase IstB	-
C5097_RS24690	61937..63298	+	1362	WP_106668545.1	DUF3560 domain-containing protein	-
C5097_RS24695	63346..63609	+	264	Protein_66	class I SAM-dependent methyltransferase	-
C5097_RS24700	63644..64348	+	705	WP_199796835.1	chromosome partitioning protein ParB	-
C5097_RS24705	64403..64837	+	435	WP_096115306.1	conjugation system SOS inhibitor PsiB	-
C5097_RS24710	64834..65553	+	720	WP_106668546.1	plasmid SOS inhibition protein A	-
C5097_RS25255	65565..65753	-	189	WP_032189914.1	hypothetical protein	-
-	65565..65789	+	225	NuclAT_0	-	-
-	65565..65789	+	225	NuclAT_0	-	-
-	65565..65789	+	225	NuclAT_0	-	-
-	65565..65789	+	225	NuclAT_0	-	-
-	65721..65784	-	64	-	-	Antitoxin
C5097_RS24715	65833..65991	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
C5097_RS25380	66226..66405	-	180	Protein_72	pilus assembly protein	-
C5097_RS25385	66654..66845	+	192	Protein_73	single-stranded DNA-binding protein	-
C5097_RS24740	67008..68012	-	1005	WP_024186362.1	IS110 family transposase	-
C5097_RS24750	68404..69423	+	1020	WP_053901803.1	IS110-like element ISEc66 family transposase	-
C5097_RS24760	69720..70217	+	498	Protein_76	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	cofA	1..106432	106432
	-	flank	IS/Tn	-	-	68404..69423	1019

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T97288 WP_001312861.1 NZ_CP027106:65833-65991 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T97288 NZ_CP027106:65833-65991 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT97288 NZ_CP027106:c65784-65721 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin