Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2206901..2207539 Replicon chromosome
Accession NZ_CP027105
Organism Escherichia coli strain RM14721

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag C5097_RS11615 Protein ID WP_000813794.1
Coordinates 2206901..2207077 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag C5097_RS11620 Protein ID WP_001270286.1
Coordinates 2207123..2207539 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5097_RS11595 2202520..2203734 - 1215 WP_071597387.1 BenE family transporter YdcO -
C5097_RS11600 2203787..2204323 + 537 WP_000429133.1 DNA-binding transcriptional regulator SutR -
C5097_RS11605 2204396..2206357 + 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
C5097_RS11610 2206449..2206679 - 231 WP_000494244.1 YncJ family protein -
C5097_RS11615 2206901..2207077 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
C5097_RS11620 2207123..2207539 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
C5097_RS11625 2207618..2209024 + 1407 WP_000760585.1 PLP-dependent aminotransferase family protein -
C5097_RS11630 2209269..2210414 + 1146 WP_106668483.1 ABC transporter substrate-binding protein -
C5097_RS11635 2210432..2211445 + 1014 WP_000220399.1 ABC transporter ATP-binding protein -
C5097_RS11640 2211446..2212387 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2201215..2202480 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T97270 WP_000813794.1 NZ_CP027105:2206901-2207077 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T97270 NZ_CP027105:2206901-2207077 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT97270 WP_001270286.1 NZ_CP027105:2207123-2207539 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT97270 NZ_CP027105:2207123-2207539 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References