Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 30772..31415 Replicon plasmid IncFIB
Accession NZ_CP027056
Organism Klebsiella pneumoniae strain 2_GR_12

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag A6D68_RS30050 Protein ID WP_001044770.1
Coordinates 30772..31188 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag A6D68_RS30055 Protein ID WP_001261282.1
Coordinates 31185..31415 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A6D68_RS30015 26314..26550 - 237 WP_000993386.1 broad-spectrum mercury transporter MerE -
A6D68_RS30020 26547..26909 - 363 WP_001277456.1 mercury resistance co-regulator MerD -
A6D68_RS30025 26927..28621 - 1695 WP_000105636.1 mercury(II) reductase -
A6D68_RS30030 28673..29095 - 423 WP_001340589.1 organomercurial transporter MerC -
A6D68_RS30035 29131..29241 - 111 Protein_29 mercuric transport protein periplasmic component -
A6D68_RS30040 29257..29954 - 698 Protein_30 IS1 family transposase -
A6D68_RS30045 29988..30698 - 711 WP_004152334.1 ATP-binding protein -
A6D68_RS30050 30772..31188 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
A6D68_RS30055 31185..31415 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
A6D68_RS30060 31372..31833 + 462 WP_072093212.1 hypothetical protein -
A6D68_RS30070 32320..32628 + 309 WP_004152336.1 hypothetical protein -
A6D68_RS30075 32656..32985 + 330 WP_004152337.1 hypothetical protein -
A6D68_RS30080 33011..33409 + 399 WP_004171440.1 hypothetical protein -
A6D68_RS30085 33416..33748 + 333 WP_004152339.1 hypothetical protein -
A6D68_RS30090 33748..34530 + 783 WP_004152340.1 site-specific integrase -
A6D68_RS30095 35422..35652 - 231 WP_011977773.1 hypothetical protein -
A6D68_RS30100 35744..36217 - 474 WP_004152341.1 YkgJ family cysteine cluster protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-2 / blaOXA-9 / blaTEM-1A - 1..95481 95481
- flank IS/Tn - - 36337..37605 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T97189 WP_001044770.1 NZ_CP027056:c31188-30772 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T97189 NZ_CP027056:c31188-30772 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT97189 WP_001261282.1 NZ_CP027056:c31415-31185 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT97189 NZ_CP027056:c31415-31185 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References