Detailed information of TA system TA097035

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3848087..3848877
• Replicon: chromosome
• Accession: NZ_CP027027
• Organism: Shigella dysenteriae strain E670/74

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1L4HFA4
• Locus tag: C5S64_RS21090
• Protein ID: WP_032163901.1
• Coordinates: 3848503..3848877 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: C5S64_RS21085
• Protein ID: WP_170936813.1
• Coordinates: 3848087..3848413 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5S64_RS21050	3844964..3845419	+	456	WP_000581504.1	hypothetical protein	-
C5S64_RS21055	3845498..3845731	+	234	WP_001119729.1	DUF905 family protein	-
C5S64_RS21065	3845831..3846649	+	819	WP_001234630.1	DUF945 domain-containing protein	-
C5S64_RS21070	3846704..3847189	+	486	WP_000849582.1	antirestriction protein	-
C5S64_RS21075	3847205..3847681	+	477	WP_001186725.1	RadC family protein	-
C5S64_RS21080	3847750..3847971	+	222	WP_000692318.1	DUF987 domain-containing protein	-
C5S64_RS21085	3848087..3848413	+	327	WP_170936813.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C5S64_RS21090	3848503..3848877	+	375	WP_032163901.1	TA system toxin CbtA family protein	Toxin
C5S64_RS21095	3848874..3849053	+	180	WP_053902470.1	hypothetical protein	-
C5S64_RS28945	3849066..3849179	+	114	WP_001161660.1	DUF957 domain-containing protein	-
C5S64_RS21105	3849468..3849596	-	129	Protein_3812	transposase domain-containing protein	-
C5S64_RS21110	3849668..3849850	+	183	WP_001016348.1	hypothetical protein	-
C5S64_RS21115	3849951..3850280	-	330	WP_000450409.1	DUF496 family protein	-
C5S64_RS21120	3850452..3851510	-	1059	WP_001200910.1	FUSC family protein	-
C5S64_RS21125	3851708..3852181	-	474	WP_001105389.1	DNA gyrase inhibitor SbmC	-
C5S64_RS21130	3852300..3853466	-	1167	WP_001296209.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13919.89 Da
• Isoelectric Point: 7.7760

>T97035 WP_032163901.1 NZ_CP027027:3848503-3848877 [Shigella dysenteriae]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T97035 NZ_CP027027:3848503-3848877 [Shigella dysenteriae]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12007.84 Da
• Isoelectric Point: 7.7960

>AT97035 WP_170936813.1 NZ_CP027027:3848087-3848413 [Shigella dysenteriae]
ITPPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDTDAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYA
KGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 327 bp

>AT97035 NZ_CP027027:3848087-3848413 [Shigella dysenteriae]
ATCACGCCCCCCTGGTGGGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTT
GCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGTTCAGCGATACAGATGCGTACCACCTGGACCAGGCCTTTCCGC
TGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGTGAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCA
AAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGGTGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAA
AAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1L4HFA4