Detailed information of TA system TA097018

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-agrB/-
• Location: 1803847..1804257
• Replicon: chromosome
• Accession: NZ_CP027027
• Organism: Shigella dysenteriae strain E670/74

Toxin (Protein)

• Gene name: hok
• Uniprot ID: Q2VNZ0
• Locus tag: C5S64_RS09470
• Protein ID: WP_000775238.1
• Coordinates: 1803847..1804008 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	1804054..1804257 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5S64_RS09440	1799020..1799694	+	675	WP_001088287.1	IS66 family insertion sequence hypothetical protein	-
C5S64_RS09445	1799691..1800038	+	348	WP_000631709.1	IS66 family insertion sequence element accessory protein TnpB	-
C5S64_RS09450	1800058..1801658	+	1601	Protein_1707	IS66 family transposase	-
C5S64_RS09455	1801658..1801858	+	201	WP_105241855.1	transposase	-
C5S64_RS09460	1801862..1803395	+	1534	WP_105241856.1	IS3 family transposase	-
C5S64_RS09470	1803847..1804008	-	162	WP_000775238.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	1804054..1804257	+	204	-	-	Antitoxin
-	1804054..1804260	-	207	NuclAT_2	-	-
-	1804054..1804260	-	207	NuclAT_2	-	-
-	1804054..1804260	-	207	NuclAT_2	-	-
-	1804054..1804260	-	207	NuclAT_2	-	-
C5S64_RS09485	1804528..1805469	-	942	Protein_1711	IS66 family transposase	-
C5S64_RS09490	1805489..1805836	-	348	WP_000622995.1	IS66 family insertion sequence element accessory protein TnpB	-
C5S64_RS09495	1805836..1806213	-	378	Protein_1713	transposase	-
C5S64_RS09500	1806280..1807290	+	1011	WP_171765179.1	IS4 family transposase	-
C5S64_RS09505	1807481..1808239	+	759	WP_004996908.1	(2E,6E)-farnesyl-diphosphate-specific ditrans,polycis-undecaprenyl-diphosphate synthase	-
C5S64_RS09510	1808252..1809109	+	858	WP_000922455.1	phosphatidate cytidylyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	1797470..1806141	8671

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T97018 WP_000775238.1 NZ_CP027027:c1804008-1803847 [Shigella dysenteriae]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

>T97018 NZ_CP027027:c1804008-1803847 [Shigella dysenteriae]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACATTGTTGATATTCACACTCCTGACCCG
GAATCGCCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAAGTTACGGCAACAATGGCTTACGAATCCGGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 204 bp

>AT97018 NZ_CP027027:1804054-1804257 [Shigella dysenteriae]
GTGGACTAGACATGCAGAGGCCTCGTGGGTTAATGAAAATTAACTACGGGGCTTTTGTCCTTCTGCCACACAACAGGATA
ACAAACCACCTTCACGTCATGAGGCAGAAAGCCTCAAGCGCCGGGCACATCATAGCCCATATACCTGCACGCTGACCACA
CTCACTTTCCCTGAAAATAATCTGGTCGTTCAGCCAGTTCACGG

Structures

Toxin

• AlphaFold DB: A0A7Z7YI12

Antitoxin