Detailed information of TA system TA096690

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 59992..60246
• Replicon: plasmid pCFS3273-2
• Accession: NZ_CP026934
• Organism: Escherichia coli strain CFS3273

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: C5F73_RS26275
• Protein ID: WP_001553767.1
• Coordinates: 59992..60198 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	60185..60246 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5F73_RS26230	55003..55182	-	180	Protein_63	hypothetical protein	-
C5F73_RS26235	55534..55788	+	255	WP_000343103.1	hypothetical protein	-
C5F73_RS26240	55788..56378	+	591	WP_000194550.1	hypothetical protein	-
C5F73_RS26245	56396..56743	-	348	WP_001115718.1	hypothetical protein	-
C5F73_RS26250	56828..57109	-	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C5F73_RS26255	57106..57375	-	270	WP_000079928.1	hypothetical protein	-
C5F73_RS26260	58289..59146	-	858	WP_000131010.1	incFII family plasmid replication initiator RepA	-
C5F73_RS26265	59139..59213	-	75	WP_001365705.1	RepA leader peptide Tap	-
C5F73_RS26270	59460..59708	-	249	WP_000083835.1	replication regulatory protein RepA	-
C5F73_RS26275	59992..60198	-	207	WP_001553767.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	60185..60246	+	62	NuclAT_2	-	Antitoxin
-	60185..60246	+	62	NuclAT_2	-	Antitoxin
-	60185..60246	+	62	NuclAT_2	-	Antitoxin
-	60185..60246	+	62	NuclAT_2	-	Antitoxin
C5F73_RS26280	60421..60803	-	383	Protein_73	hypothetical protein	-
C5F73_RS26285	60906..61367	-	462	WP_000760078.1	thermonuclease family protein	-
C5F73_RS26290	62110..62313	-	204	WP_001327131.1	hypothetical protein	-
C5F73_RS26295	62468..63025	-	558	WP_000139312.1	fertility inhibition protein FinO	-
C5F73_RS26300	63128..63988	-	861	WP_000704512.1	alpha/beta hydrolase	-
C5F73_RS26305	64047..64793	-	747	WP_000205745.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / tet(B) / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / sul2 / sitABCD	iutA / iucD / iucC / iucB / iucA	1..145001	145001

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7804.35 Da
• Isoelectric Point: 8.8807

>T96690 WP_001553767.1 NZ_CP026934:c60198-59992 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYALIGVLAVCATVLCFLLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T96690 NZ_CP026934:c60198-59992 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GGTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTTACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT96690 NZ_CP026934:60185-60246 [Escherichia coli]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin