Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1857869..1858487 Replicon chromosome
Accession NZ_CP026932
Organism Escherichia coli strain CFS3273

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C5F73_RS08975 Protein ID WP_001291435.1
Coordinates 1857869..1858087 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C5F73_RS08980 Protein ID WP_000344800.1
Coordinates 1858113..1858487 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5F73_RS08940 1853158..1853730 + 573 WP_000779831.1 YbaY family lipoprotein -
C5F73_RS08945 1853761..1854150 - 390 WP_001300427.1 DNA base-flipping protein -
C5F73_RS08955 1854451..1854804 + 354 WP_000878140.1 DUF1428 domain-containing protein -
C5F73_RS08960 1854846..1856396 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C5F73_RS08965 1856560..1857030 - 471 WP_000136192.1 YlaC family protein -
C5F73_RS08970 1857146..1857697 - 552 WP_000102564.1 maltose O-acetyltransferase -
C5F73_RS08975 1857869..1858087 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
C5F73_RS08980 1858113..1858487 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C5F73_RS08985 1859033..1862182 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
C5F73_RS08990 1862205..1863398 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T96670 WP_001291435.1 NZ_CP026932:c1858087-1857869 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T96670 NZ_CP026932:c1858087-1857869 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT96670 WP_000344800.1 NZ_CP026932:c1858487-1858113 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT96670 NZ_CP026932:c1858487-1858113 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References