Detailed information of TA system TA096532

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 4665953..4666549
• Replicon: chromosome
• Accession: NZ_CP026880
• Organism: Pseudomonas sp. LH1G9

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C5F70_RS21660
• Protein ID: WP_079442744.1
• Coordinates: 4665953..4666255 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1D3JR09
• Locus tag: C5F70_RS21665
• Protein ID: WP_017849687.1
• Coordinates: 4666259..4666549 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5F70_RS21640	4661665..4662291	-	627	WP_017849678.1	precorrin-8X methylmutase	-
C5F70_RS21645	4662284..4663636	-	1353	WP_104904026.1	precorrin-3B synthase	-
C5F70_RS21650	4663675..4664880	+	1206	WP_104904027.1	precorrin-6y C5,15-methyltransferase (decarboxylating) subunit CbiE	-
C5F70_RS21655	4664877..4665596	+	720	WP_057006059.1	cobalt-precorrin-6A reductase	-
C5F70_RS21660	4665953..4666255	+	303	WP_079442744.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C5F70_RS21665	4666259..4666549	+	291	WP_017849687.1	putative addiction module antidote protein	Antitoxin
C5F70_RS21670	4666646..4667041	+	396	WP_046485671.1	DUF2946 domain-containing protein	-
C5F70_RS21675	4667089..4667571	+	483	WP_046384861.1	copper chaperone PCu(A)C	-
C5F70_RS21680	4667583..4668008	+	426	WP_079442743.1	DUF2946 domain-containing protein	-
C5F70_RS21685	4668102..4670171	+	2070	WP_104904028.1	TonB-dependent copper receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Uncharacterized protein HI1420 (5-94)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11549.43 Da
• Isoelectric Point: 9.1221

>T96532 WP_079442744.1 NZ_CP026880:4665953-4666255 [Pseudomonas sp. LH1G9]
MIEFEESIEFVKWLEAMRDPLAKVRIVTRLRMAQAGNFGDCEAVGEGVCEMRIHQGPGYRVYFTRRDKVVYLLLMGGDKS
TQSRDIQRAIHMAKNLGRKE

Nucleotide

• Length: 303 bp

>T96532 NZ_CP026880:4665953-4666255 [Pseudomonas sp. LH1G9]
ATGATTGAGTTCGAAGAATCCATTGAGTTTGTCAAATGGCTCGAAGCAATGCGCGATCCGCTCGCAAAAGTACGCATTGT
GACAAGGCTGCGCATGGCGCAAGCAGGAAACTTCGGCGACTGTGAGGCTGTAGGAGAGGGCGTTTGCGAAATGCGAATCC
ACCAAGGCCCCGGGTATCGGGTTTACTTCACTCGCCGAGACAAGGTGGTTTATCTGCTGCTGATGGGCGGCGACAAATCA
ACACAAAGCCGAGACATCCAGCGTGCAATACACATGGCAAAAAATCTTGGCCGCAAGGAGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10437.00 Da
• Isoelectric Point: 5.8124

>AT96532 WP_017849687.1 NZ_CP026880:4666259-4666549 [Pseudomonas sp. LH1G9]
MSESFTRFDAAKYLKTPFEMAAYLDACFEEDAGDGLLIRTALNDIARAQGMTQVARDAGLGRESLYKALSSTGNPEFATI
MKVMRALGLKLHATAL

Nucleotide

• Length: 291 bp

>AT96532 NZ_CP026880:4666259-4666549 [Pseudomonas sp. LH1G9]
ATGAGCGAATCCTTTACCCGTTTCGATGCCGCAAAATATCTCAAGACTCCTTTTGAGATGGCCGCCTATCTGGATGCTTG
CTTTGAAGAAGATGCTGGCGACGGTCTGTTGATTCGCACAGCGCTGAACGATATCGCTCGCGCCCAAGGCATGACCCAAG
TCGCTCGCGACGCCGGCCTGGGGCGAGAAAGTTTGTACAAAGCCCTCTCCAGCACTGGCAATCCCGAGTTCGCGACTATC
ATGAAGGTCATGAGAGCACTGGGGCTCAAACTTCACGCCACCGCGCTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1D3JR09