Detailed information of TA system TA096435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1744276..1744865
• Replicon: chromosome
• Accession: NZ_CP026846
• Organism: Shigella boydii strain 59-2708

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A398TMA1
• Locus tag: C1P57_RS08610
• Protein ID: WP_000513533.1
• Coordinates: 1744276..1744524 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C1P57_RS08615
• Protein ID: WP_001016018.1
• Coordinates: 1744521..1744865 (+)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P57_RS08580 (1739853)	1739853..1740791	-	939	Protein_1665	tyrosine-type recombinase/integrase	-
C1P57_RS08590 (1742039)	1742039..1742134	-	96	WP_284522451.1	host nuclease inhibitor GamL	-
C1P57_RS08595 (1742155)	1742155..1742436	-	282	WP_000934193.1	hypothetical protein	-
C1P57_RS08605 (1742731)	1742731..1743621	-	891	WP_078167383.1	Rha family phage regulatory protein	-
C1P57_RS08610 (1744276)	1744276..1744524	+	249	WP_000513533.1	type II toxin-antitoxin system HicA family toxin	Toxin
C1P57_RS08615 (1744521)	1744521..1744865	+	345	WP_001016018.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C1P57_RS24705 (1744980)	1744980..1745150	-	171	WP_164834428.1	hypothetical protein	-
C1P57_RS08620 (1745272)	1745272..1745990	-	719	Protein_1673	LexA family transcriptional regulator	-
C1P57_RS08625 (1746096)	1746096..1746335	+	240	WP_000171144.1	helix-turn-helix transcriptional regulator	-
C1P57_RS25185 (1746382)	1746382..1746639	+	258	Protein_1675	hypothetical protein	-
C1P57_RS08635 (1746861)	1746861..1747067	+	207	WP_284522452.1	hypothetical protein	-
C1P57_RS08640 (1747124)	1747124..1747746	+	623	Protein_1677	IS3-like element IS911 family transposase	-
C1P57_RS08645 (1747741)	1747741..1748416	+	676	Protein_1678	IclR family transcriptional regulator	-
C1P57_RS08650 (1748431)	1748431..1749636	+	1206	WP_000174592.1	L-rhamnonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1738436..1769115	30679
	-	flank	IS/Tn	-	-	1747124..1747426	302

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9436.07 Da
• Isoelectric Point: 10.3195

>T96435 WP_000513533.1 NZ_CP026846:1744276-1744524 [Shigella boydii]
MGKADKLLEKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Nucleotide

• Length: 249 bp

>T96435 NZ_CP026846:1744276-1744524 [Shigella boydii]
ATGGGGAAAGCAGATAAGCTACTGGAAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAGTTGGTCGTTTTGTT
TTCCTCTCTGGGATATGTCAAAAAGGAAATGCAGGGCTCAAGAGTGCGGTTTTTCAATGCTGAAATCAACCACACCATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAGCGATTAAACAGAATCTGAAAGAGGCTGGG
GTATTATGA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 13051.73 Da
• Isoelectric Point: 4.5590

>AT96435 WP_001016018.1 NZ_CP026846:1744521-1744865 [Shigella boydii]
MNHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEASTLAELEQEFKTSVELYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVNHHTGA

Nucleotide

• Length: 345 bp

>AT96435 NZ_CP026846:1744521-1744865 [Shigella boydii]
ATGAATCATCTAAAATATAAAGGATATTTAGGTACAGTAGAACCAGATTTTGAAAATAATATCCTGTACGGAAAACTGGC
GTTTATTCGAGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAGACATCGGTTGAGCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAGCCTGATACCCCGTTTAAAGGTGTGTTTAACGTCAGACTTGATCCAGAA
CTGCATCGTCGGGTCGCTGAAATGGCGATGGAAGAAGATTTATCACTGAACGCCTTTGTTAATAAGGCGCTGGAAAAAGA
AGTCAACCATCACACAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A398TMA1