Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 21052..21577 Replicon plasmid unnamed
Accession NZ_CP026837
Organism Shigella boydii strain ATCC 49812

Toxin (Protein)


Gene name ccdB Uniprot ID Q326Z8
Locus tag C1P56_RS26065 Protein ID WP_001159860.1
Coordinates 21052..21357 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q7BEK0
Locus tag C1P56_RS26070 Protein ID WP_000813626.1
Coordinates 21359..21577 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P56_RS26035 16548..17138 - 591 WP_000705601.1 type III secretion system effector protein kinase OspG -
C1P56_RS28775 17221..17390 - 170 Protein_20 type II toxin-antitoxin system toxin YacB -
C1P56_RS26045 17390..17659 - 270 WP_000079956.1 type II toxin-antitoxin system antitoxin YacA -
C1P56_RS26050 17867..19504 - 1638 WP_134796740.1 T3SS effector E3 ubiquitin-protein ligase IpaH9.8 -
C1P56_RS26055 19766..19873 - 108 WP_023592908.1 transposase domain-containing protein -
C1P56_RS26060 19989..21020 + 1032 WP_001121440.1 IS630-like element IS630 family transposase -
C1P56_RS26065 21052..21357 - 306 WP_001159860.1 type II toxin-antitoxin system toxin CcdB Toxin
C1P56_RS26070 21359..21577 - 219 WP_000813626.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C1P56_RS26075 22113..23204 - 1092 Protein_27 IS66 family transposase -
C1P56_RS26080 23212..23781 + 570 Protein_28 plasmid segregation protein ParM -
C1P56_RS26085 23784..24134 + 351 WP_134796741.1 plasmid partitioning/stability family protein -
C1P56_RS27965 24241..24378 + 138 WP_000766699.1 hypothetical protein -
C1P56_RS26090 24510..24785 - 276 WP_000421262.1 type II toxin-antitoxin system RelE/ParE family toxin -
C1P56_RS26095 24785..25069 - 285 WP_001178088.1 ribbon-helix-helix domain-containing protein -
C1P56_RS26105 25521..25772 + 252 WP_000433928.1 transporter -
C1P56_RS26110 25822..26031 + 210 Protein_34 peptidoglycan-binding protein -
C1P56_RS26115 26131..26316 - 186 Protein_35 ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - ospG / ipaH9.8 / ospI / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ospD3/senA / ospC1 / faeI / faeI / faeH / faeF / faeE / faeD / faeC / ipgB2 / ospD1 / ospF / ospD2 / ospC2 / ospC4 / ospC3 / ospB / icsP/sopA / nleE / ospC2 1..213410 213410
- inside IScluster/Tn - ospG / ipaH9.8 / ospI 14515..35518 21003


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11736.59 Da        Isoelectric Point: 6.4674

>T96385 WP_001159860.1 NZ_CP026837:c21357-21052 [Shigella boydii]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T96385 NZ_CP026837:c21357-21052 [Shigella boydii]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGACGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACTGATATGGCCAGTGTGCCGATCTTCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8333.36 Da        Isoelectric Point: 6.2838

>AT96385 WP_000813626.1 NZ_CP026837:c21577-21359 [Shigella boydii]
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT96385 NZ_CP026837:c21577-21359 [Shigella boydii]
ATGAAGCAGCGTATTACTGTCACTATAGACAGTGACAGCTATCAGTTACTTAAATCCGCAAACGTGAATATCTCAGGCCT
GGTAAATACGGCCATGCAAAAAGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCTGAAAATCAGCAGGGGATGGCTG
AGATCGCCCGTTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTN3

Antitoxin

Source ID Structure
AlphaFold DB Q7BEK0

References