Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2037639..2038333 Replicon chromosome
Accession NZ_CP026788
Organism Shigella flexneri 2a strain ATCC 29903

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag C1P79_RS10915 Protein ID WP_001263491.1
Coordinates 2037639..2038037 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag C1P79_RS10920 Protein ID WP_000554759.1
Coordinates 2038040..2038333 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P79_RS10890 (2032978) 2032978..2033436 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
C1P79_RS10895 (2033698) 2033698..2035155 + 1458 WP_001293030.1 cytosol nonspecific dipeptidase -
C1P79_RS10900 (2035212) 2035212..2035772 - 561 Protein_2074 peptide chain release factor H -
C1P79_RS10905 (2035888) 2035888..2036931 - 1044 WP_005053205.1 RNA ligase RtcB family protein -
C1P79_RS10910 (2037177) 2037177..2037629 - 453 WP_001059860.1 GNAT family N-acetyltransferase -
C1P79_RS10915 (2037639) 2037639..2038037 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
C1P79_RS10920 (2038040) 2038040..2038333 - 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
C1P79_RS10925 (2038385) 2038385..2039440 - 1056 WP_001226172.1 DNA polymerase IV -
C1P79_RS10930 (2039511) 2039511..2040296 - 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
C1P79_RS10935 (2040268) 2040268..2041980 + 1713 Protein_2081 flagellar biosynthesis protein FlhA -
C1P79_RS10940 (2042197) 2042197..2042694 - 498 WP_000006251.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gtrII / gtrB / gtrA 2007531..2039440 31909


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T95946 WP_001263491.1 NZ_CP026788:c2038037-2037639 [Shigella flexneri 2a]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T95946 NZ_CP026788:c2038037-2037639 [Shigella flexneri 2a]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT95946 WP_000554759.1 NZ_CP026788:c2038333-2038040 [Shigella flexneri 2a]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT95946 NZ_CP026788:c2038333-2038040 [Shigella flexneri 2a]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
AAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References