Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 20619..21246 Replicon chromosome
Accession NZ_CP026777
Organism Shigella dysenteriae strain 69-3818

Toxin (Protein)


Gene name higB Uniprot ID A0A828BKW8
Locus tag C5C01_RS00155 Protein ID WP_024258354.1
Coordinates 20619..20948 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID Q32DW6
Locus tag C5C01_RS00160 Protein ID WP_000147081.1
Coordinates 20938..21246 (+) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5C01_RS00115 15884..16600 + 717 WP_001001693.1 arginine ABC transporter permease ArtQ -
C5C01_RS00120 16600..17268 + 669 WP_000464490.1 arginine ABC transporter permease ArtM -
C5C01_RS00125 17559..17879 + 321 Protein_21 ABC transporter substrate-binding protein ArtJ -
C5C01_RS00140 18646..18786 - 141 Protein_23 HNH endonuclease -
C5C01_RS00145 18824..19546 - 723 WP_001004003.1 DNA-binding protein -
C5C01_RS00150 19621..20298 - 678 WP_000957540.1 phage antirepressor Ant -
C5C01_RS00155 20619..20948 + 330 WP_024258354.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C5C01_RS00160 20938..21246 + 309 WP_000147081.1 helix-turn-helix transcriptional regulator Antitoxin
C5C01_RS00165 21243..21464 - 222 WP_001254231.1 NinE family protein -
C5C01_RS00170 21461..21871 - 411 WP_000814621.1 recombination protein NinB -
C5C01_RS00180 23289..24293 + 1005 Protein_31 tyrosine-type recombinase/integrase -
C5C01_RS00185 24351..24911 - 561 WP_001215754.1 DUF1175 domain-containing protein -
C5C01_RS00190 24908..25249 - 342 Protein_33 DUF2138 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 6889..56130 49241
- inside IScluster/Tn - - 10739..23155 12416


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-97)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12837.87 Da        Isoelectric Point: 9.9966

>T95827 WP_024258354.1 NZ_CP026777:20619-20948 [Shigella dysenteriae]
MNYTIEYYSEDVRLEVDRLPLSMRARYQHLVERMKIYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIML
HSFVKKTQKTPPAERKKAETRMKEVKHDW

Download         Length: 330 bp

>T95827 NZ_CP026777:20619-20948 [Shigella dysenteriae]
ATGAACTACACTATCGAATACTACAGTGAAGATGTAAGGCTGGAAGTCGATCGGCTTCCATTGAGTATGCGTGCCCGATA
CCAACATCTCGTTGAACGTATGAAGATATATGGCAGCAATCTCGGAGAACCTCACACCAGCGCCTTTGGTGACGGACTTT
TCGAACTCAGAATTAAAGGCAACGATGGGATCGCGCGTGTTTTTTACTGCACTCTGACAGGGAAACGCATCATCATGCTG
CATAGTTTTGTAAAGAAAACGCAGAAAACACCGCCAGCCGAACGCAAGAAAGCTGAAACCAGAATGAAGGAGGTTAAGCA
TGACTGGTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11185.94 Da        Isoelectric Point: 8.5840

>AT95827 WP_000147081.1 NZ_CP026777:20938-21246 [Shigella dysenteriae]
MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGISG
PSFATLKKFARATGKKLQIRFV

Download         Length: 309 bp

>AT95827 NZ_CP026777:20938-21246 [Shigella dysenteriae]
ATGACTGGTAAACGCACTCTCCCCACCATGACACACGACGAAATGGCAGCCAAATGGATGGAAGACCCGGCTTTTAAAGC
AGAATACGACGCTATCGCTGACGAATTCGCACTGCTTGATGAAATGCTGGCAGCACGCAAAGAAGCTGGCTTAACTCAGG
CTGAAGTTGCCGAGCGAATGGGAACAAAAGCGACCGCGATCACCAGAATGGAAAGTAATCTCGCATCAGGTATCAGCGGC
CCATCATTTGCCACCCTAAAAAAATTCGCCCGCGCTACTGGAAAAAAACTCCAGATCCGCTTCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828BKW8

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TJC1

References