95820

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1934461..1935305 Replicon chromosome
Accession NZ_CP026776
Organism Shigella flexneri strain 98-3193

Toxin (Protein)


Gene name itaT Uniprot ID A0A4P7TPN5
Locus tag C1P84_RS10445 Protein ID WP_005060796.1
Coordinates 1934907..1935305 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID A5H0K0
Locus tag C1P84_RS10435 Protein ID WP_005053053.1
Coordinates 1934461..1934772 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P84_RS10410 (1929931) 1929931..1930428 + 498 Protein_1978 COX aromatic rich motif-containing protein -
C1P84_RS10415 (1930450) 1930450..1932441 + 1992 Protein_1979 cytochrome o ubiquinol oxidase subunit I -
C1P84_RS10420 (1932431) 1932431..1933045 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
C1P84_RS10425 (1933045) 1933045..1933374 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
C1P84_RS10430 (1933386) 1933386..1934276 + 891 WP_000971356.1 heme o synthase -
C1P84_RS10435 (1934461) 1934461..1934772 + 312 WP_005053053.1 DUF1778 domain-containing protein Antitoxin
C1P84_RS10440 (1934756) 1934756..1934914 + 159 WP_005053055.1 hypothetical protein -
C1P84_RS10445 (1934907) 1934907..1935305 + 399 WP_005060796.1 GNAT family N-acetyltransferase Toxin
C1P84_RS10455 (1936201) 1936201..1938354 + 2154 WP_005060811.1 SEL1-like repeat protein -
C1P84_RS10460 (1938407) 1938407..1940137 + 1731 WP_000645011.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1935353..1935856 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(52-112)

Antitoxin

Vibrio phage ICP1, Orf50

(15-97)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14335.69 Da        Isoelectric Point: 9.1674

>T95820 WP_005060796.1 NZ_CP026776:1934907-1935305 [Shigella flexneri]
VRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKK
IHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA

Download         Length: 399 bp

>T95820 NZ_CP026776:1934907-1935305 [Shigella flexneri]
GTGCGTAATAGCGACTGTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGC
ATATTCGCTGGAAAGACAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCA
TGTTGGGGGGAGCGCGGAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAAAA
ATTCACCCGGCATTACCAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTT
TGTTCAGCTTTCAGCGACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11394.07 Da        Isoelectric Point: 10.3388

>AT95820 WP_005053053.1 NZ_CP026776:1934461-1934772 [Shigella flexneri]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT95820 NZ_CP026776:1934461-1934772 [Shigella flexneri]
ATGCCTGGAAAAACAGCCGCTCTATACGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPN5

Antitoxin

Source ID Structure
AlphaFold DB A5H0K0

References