Detailed information of TA system TA095717

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1068074..1068602
• Replicon: chromosome
• Accession: NZ_CP026765
• Organism: Shigella flexneri strain 04-3145

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C1P85_RS05760
• Protein ID: WP_005061113.1
• Coordinates: 1068372..1068602 (-)
• Length: 77 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q32DW6
• Locus tag: C1P85_RS05755
• Protein ID: WP_000147081.1
• Coordinates: 1068074..1068382 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P85_RS05715 (1064132)	1064132..1064365	+	234	Protein_1034	DUF1367 family protein	-
C1P85_RS05720 (1064365)	1064365..1064655	+	291	WP_000228005.1	DUF1364 domain-containing protein	-
C1P85_RS05725 (1064652)	1064652..1064762	+	111	Protein_1036	DUF1133 family protein	-
C1P85_RS05730 (1064839)	1064839..1066007	+	1169	WP_000747032.1	IS3-like element IS911 family transposase	-
C1P85_RS05735 (1066332)	1066332..1067099	+	768	Protein_1038	hypothetical protein	-
C1P85_RS05740 (1067174)	1067174..1067452	+	279	WP_005061104.1	hypothetical protein	-
C1P85_RS05745 (1067449)	1067449..1067859	+	411	WP_000814621.1	recombination protein NinB	-
C1P85_RS05750 (1067856)	1067856..1068032	+	177	WP_024260406.1	NinE family protein	-
C1P85_RS05755 (1068074)	1068074..1068382	-	309	WP_000147081.1	helix-turn-helix transcriptional regulator	Antitoxin
C1P85_RS05760 (1068372)	1068372..1068602	-	231	WP_005061113.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C1P85_RS05765 (1068615)	1068615..1069073	+	459	WP_000961311.1	ash family protein	-
C1P85_RS05770 (1069131)	1069131..1069583	+	453	WP_232046050.1	Rha family transcriptional regulator	-
C1P85_RS05775 (1069580)	1069580..1069813	+	234	WP_000618004.1	hypothetical protein	-
C1P85_RS05780 (1069800)	1069800..1070708	+	909	WP_001247840.1	hypothetical protein	-
C1P85_RS05785 (1070719)	1070719..1071597	+	879	WP_000988183.1	ATP-binding protein	-
C1P85_RS05790 (1071594)	1071594..1072985	+	1392	WP_000211324.1	replicative DNA helicase	-
C1P85_RS05795 (1072982)	1072982..1073239	+	258	WP_001064799.1	hypothetical protein	-
C1P85_RS05800 (1073334)	1073334..1073534	+	201	WP_000111769.1	protein ninH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1045348..1105460	60112

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (5-70)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-97)

Sequences

Toxin

Protein

• Length: 77 a.a.
• Molecular weight: 8665.12 Da
• Isoelectric Point: 10.5308

>T95717 WP_005061113.1 NZ_CP026765:c1068602-1068372 [Shigella flexneri]
MKVYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKAQKTPPAERKKAETRMKEVKHDW

Nucleotide

• Length: 231 bp

>T95717 NZ_CP026765:c1068602-1068372 [Shigella flexneri]
ATGAAGGTATACGGCAGCAATCTCGGAGAACCTCACACCAGCGCCTTTGGTGACGGGCTTTTCGAACTCAGAATTAAAGG
CAACGATGGGATCGCGCGTGTTTTTTACTGCACTCTGACAGGGAAACGCATCATCATGCTGCATAGTTTTGTAAAGAAAG
CGCAGAAAACACCGCCAGCCGAACGCAAGAAAGCTGAAACCAGAATGAAGGAGGTTAAGCATGACTGGTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11185.94 Da
• Isoelectric Point: 8.5840

>AT95717 WP_000147081.1 NZ_CP026765:c1068382-1068074 [Shigella flexneri]
MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGISG
PSFATLKKFARATGKKLQIRFV

Nucleotide

• Length: 309 bp

>AT95717 NZ_CP026765:c1068382-1068074 [Shigella flexneri]
ATGACTGGTAAACGCACTCTCCCCACCATGACACACGACGAAATGGCAGCCAAATGGATGGAAGACCCGGCTTTTAAAGC
AGAATACGACGCTATCGCTGACGAATTCGCACTGCTTGATGAAATGCTGGCAGCACGCAAAGAAGCTGGCTTAACTCAGG
CTGAAGTTGCCGAGCGAATGGGAACAAAAGCGACCGCGATCACCAGAATGGAAAGTAATCTCGCATCAGGTATCAGCGGC
CCATCATTTGCCACACTAAAAAAATTCGCCCGCGCTACTGGAAAAAAACTCCAGATCCGCTTCGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A4P7TJC1