Detailed information of TA system TA095715

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 698039..698883
• Replicon: chromosome
• Accession: NZ_CP026765
• Organism: Shigella flexneri strain 04-3145

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: C1P85_RS03820
• Protein ID: WP_252981315.1
• Coordinates: 698039..698500 (-)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: A5H0K0
• Locus tag: C1P85_RS03830
• Protein ID: WP_005053053.1
• Coordinates: 698572..698883 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P85_RS03805 (693207)	693207..694937	-	1731	WP_128882009.1	hypothetical protein	-
C1P85_RS03810 (694990)	694990..697143	-	2154	WP_128882010.1	SEL1-like repeat protein	-
C1P85_RS03815 (697294)	697294..697991	+	698	WP_223368647.1	IS1 family transposase	-
C1P85_RS03820 (698039)	698039..698500	-	462	WP_252981315.1	GNAT family N-acetyltransferase	Toxin
C1P85_RS03830 (698572)	698572..698883	-	312	WP_005053053.1	DUF1778 domain-containing protein	Antitoxin
C1P85_RS03835 (699068)	699068..699958	-	891	WP_000971346.1	heme o synthase	-
C1P85_RS03840 (699970)	699970..700299	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
C1P85_RS03845 (700299)	700299..700913	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
C1P85_RS03850 (700903)	700903..702894	-	1992	Protein_681	cytochrome o ubiquinol oxidase subunit I	-
C1P85_RS03855 (702916)	702916..703413	-	498	Protein_682	COX aromatic rich motif-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP037923 Shigella flexneri 5a str. M90T 4168367..4168870 0.004	flank	IS/Tn	-	-	697488..697991	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (62-133)

Antitoxin

• Vibrio phage ICP1, Orf50 (15-97)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16966.80 Da
• Isoelectric Point: 9.9662

>T95715 WP_252981315.1 NZ_CP026765:c698500-698039 [Shigella flexneri]
MIAVTLLSINLYALRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSWRPSGFTTFRNWCCQISHVGGAR
KYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 462 bp

>T95715 NZ_CP026765:c698500-698039 [Shigella flexneri]
TTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACTG
TGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAGAC
AACGCGTTTCTTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGAGCGCGG
AAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAAAAATTCACCCGGCATTACC
AATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCGA
CACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11394.07 Da
• Isoelectric Point: 10.3388

>AT95715 WP_005053053.1 NZ_CP026765:c698883-698572 [Shigella flexneri]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT95715 NZ_CP026765:c698883-698572 [Shigella flexneri]
ATGCCTGGAAAAACAGCCGCTCTATACGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A5H0K0