Detailed information of TA system TA095582

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 4705103..4705808
• Replicon: chromosome
• Accession: NZ_CP026723
• Organism: Escherichia coli strain 266917_2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: C4R22_RS24020
• Protein ID: WP_000539521.1
• Coordinates: 4705422..4705808 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C4R22_RS24015
• Protein ID: WP_001280945.1
• Coordinates: 4705103..4705432 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4R22_RS24000	4700260..4701171	+	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-
C4R22_RS24005	4701349..4703697	+	2349	WP_001550886.1	EAL domain-containing protein	-
C4R22_RS24010	4703705..4705033	+	1329	WP_001550887.1	GGDEF domain-containing protein	-
C4R22_RS24015	4705103..4705432	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
C4R22_RS24020	4705422..4705808	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4R22_RS24025	4706034..4707359	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
C4R22_RS24030	4707572..4707955	+	384	WP_000497137.1	biofilm formation regulator BssR	-
C4R22_RS24035	4708066..4709181	+	1116	WP_000555051.1	aldose sugar dehydrogenase YliI	-
C4R22_RS24040	4709178..4709804	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T95582 WP_000539521.1 NZ_CP026723:c4705808-4705422 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T95582 NZ_CP026723:c4705808-4705422 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT95582 WP_001280945.1 NZ_CP026723:c4705432-4705103 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT95582 NZ_CP026723:c4705432-4705103 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6