Detailed information of TA system TA095219

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1718465..1718685
• Replicon: chromosome
• Accession: NZ_CP026594
• Organism: Clostridioides difficile strain 08-00495

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: Q183Z5
• Locus tag: C3348_RS08340
• Protein ID: WP_003429855.1
• Coordinates: 1718465..1718626 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	1718546..1718685 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3348_RS08305	1714322..1714759	+	438	WP_009888839.1	hypothetical protein	-
C3348_RS08310	1714752..1714928	+	177	WP_009888840.1	hypothetical protein	-
C3348_RS08315	1714929..1716239	+	1311	WP_009893136.1	phage tail sheath family protein	-
C3348_RS08320	1716256..1716726	+	471	WP_009888842.1	phage tail tube protein	-
C3348_RS08325	1716785..1717612	+	828	WP_009888843.1	hypothetical protein	-
C3348_RS08330	1717684..1718124	+	441	WP_003429853.1	phage portal protein	-
C3348_RS08340	1718465..1718626	+	162	WP_003429855.1	hypothetical protein	Toxin
-	1718546..1718685	-	140	NuclAT_1	-	Antitoxin
-	1718546..1718685	-	140	NuclAT_2	-	Antitoxin
-	1719030..1719078	-	49	NuclAT_4	-	-
-	1719030..1719078	-	49	NuclAT_5	-	-
C3348_RS08345	1719963..1720151	+	189	WP_003429858.1	hypothetical protein	-
C3348_RS08350	1720270..1721136	+	867	WP_009888845.1	hypothetical protein	-
C3348_RS08360	1722001..1722528	+	528	WP_009888847.1	DUF4352 domain-containing protein	-
C3348_RS08365	1722666..1723433	+	768	WP_009888848.1	DUF4428 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_013316 Clostridium difficile R20291 Prophage TA024266 1671047..1738184 0.001	inside	Prophage	-	-	1684676..1754629	69953

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T95219 WP_003429855.1 NZ_CP026594:1718465-1718626 [Clostridioides difficile]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T95219 NZ_CP026594:1718465-1718626 [Clostridioides difficile]
ATGAATAACTTTTTACTTAATGTAATAGCTGGCGTTATTGCTAGTTTAATATTTTGCTTAATTTGTAAAGTATTTCTAAA
AGTAAAAAGCCACTCAACTCGTGGCAAGAGTAAAAGTGGCTGGGAATTTGATTTTAAAATCAAGTTCCACAAATTTAAAT
AA

Antitoxin

Nucleotide

• Length: 140 bp

>AT95219 NZ_CP026594:c1718685-1718546 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGACAGTAGAGTGAAGTTCATAATTTAAACAATTAGTAATTATTTAAATTTGTGGAACTT
GATTTTAAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVN2

Antitoxin