Detailed information of TA system TA095050

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 159007..159587
• Replicon: plasmid pSymA
• Accession: NZ_CP026526
• Organism: Sinorhizobium meliloti strain AK21

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q92XP1
• Locus tag: C3L21_RS19280
• Protein ID: WP_010968151.1
• Coordinates: 159007..159390 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: C3L21_RS19285
• Protein ID: WP_088199999.1
• Coordinates: 159387..159587 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3L21_RS19265	154247..155182	-	936	WP_014989617.1	LysR family transcriptional regulator	-
C3L21_RS19270	156179..158230	+	2052	Protein_143	S9 family peptidase	-
C3L21_RS19275	158433..158735	+	303	WP_158528641.1	hypothetical protein	-
C3L21_RS19280	159007..159390	-	384	WP_010968151.1	type II toxin-antitoxin system VapC family toxin	Toxin
C3L21_RS19285	159387..159587	-	201	WP_088199999.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
C3L21_RS19290	159878..160279	+	402	WP_014531161.1	GFA family protein	-
C3L21_RS19295	160356..161192	-	837	WP_014531162.1	helix-turn-helix transcriptional regulator	-
C3L21_RS19300	161288..161611	+	324	WP_017266777.1	DUF1127 domain-containing protein	-
C3L21_RS19305	161650..162378	+	729	WP_014531163.1	class I SAM-dependent methyltransferase	-
C3L21_RS19310	162472..162618	+	147	WP_017269032.1	hypothetical protein	-
C3L21_RS19315	162960..163268	+	309	WP_122102879.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
C3L21_RS19320	163265..163548	+	284	Protein_153	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB / htpB	1..1506823	1506823

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14292.54 Da
• Isoelectric Point: 6.9172

>T95050 WP_010968151.1 NZ_CP026526:c159390-159007 [Sinorhizobium meliloti]
VILADTSIWIDHFRHTDAELRRIIEDDRLLCHPAVIGELALGSLRERSSVIAFLMAQREALVATHQEVMMMIDRHAIFSM
GIGYTDAHLLASVLLDQRMALWTRDKRLQAAAEKAGASLHTPAHTRN

Nucleotide

• Length: 384 bp

>T95050 NZ_CP026526:c159390-159007 [Sinorhizobium meliloti]
GTGATTCTTGCCGACACTTCAATCTGGATTGATCATTTCCGGCATACCGATGCCGAGCTGCGTAGGATTATTGAGGACGA
TCGTCTCCTCTGCCATCCGGCCGTGATCGGAGAGCTTGCACTTGGCAGCCTTCGGGAACGCAGTAGCGTGATAGCATTCC
TGATGGCTCAGCGCGAAGCACTCGTCGCAACGCACCAGGAGGTCATGATGATGATCGATCGCCACGCCATTTTTAGCATG
GGGATTGGCTACACGGATGCCCATTTGCTGGCCTCGGTTCTCCTCGATCAGCGAATGGCTTTGTGGACGAGAGACAAGCG
TTTGCAGGCAGCGGCCGAAAAGGCGGGGGCGTCGCTGCACACCCCCGCCCATACGCGGAACTGA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7080.22 Da
• Isoelectric Point: 11.1107

>AT95050 WP_088199999.1 NZ_CP026526:c159587-159387 [Sinorhizobium meliloti]
MRSTINLDDTLMERAKALTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Nucleotide

• Length: 201 bp

>AT95050 NZ_CP026526:c159587-159387 [Sinorhizobium meliloti]
ATGCGATCGACTATCAACCTCGACGACACTCTCATGGAAAGGGCTAAGGCCCTAACCGGCACAAAAGAAACCGCCGCTCT
GGTCCGCCAGGCATTGGAGACGCTTGTTCGTGTGGAGTCAGGAAAACGCCTCATCGCGCTCGGGGGAACTATGCCGGATG
CGGAAGCAGCTCCACGCCGCCGGAGCGCAGCTGCCAAGTGA

Structures

Toxin

• AlphaFold DB: Q92XP1