Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 2777115..2777782 Replicon chromosome
Accession NZ_CP026351
Organism Escherichia coli strain 5A

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag C2R12_RS14505 Protein ID WP_001094400.1
Coordinates 2777453..2777782 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag C2R12_RS14500 Protein ID WP_000072690.1
Coordinates 2777115..2777432 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2R12_RS14470 2772167..2773176 - 1010 Protein_2648 arsenic transporter -
C2R12_RS14475 2773318..2775021 + 1704 WP_000896263.1 hypothetical protein -
C2R12_RS14480 2775593..2775816 + 224 Protein_2650 DUF905 domain-containing protein -
C2R12_RS14485 2775919..2776377 + 459 WP_000211841.1 antirestriction protein -
C2R12_RS14490 2776386..2776868 + 483 WP_001407480.1 RadC family protein -
C2R12_RS14495 2776877..2777077 + 201 WP_001395452.1 DUF987 domain-containing protein -
C2R12_RS14500 2777115..2777432 + 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
C2R12_RS14505 2777453..2777782 + 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
C2R12_RS14510 2778146..2782726 - 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T94260 WP_001094400.1 NZ_CP026351:2777453-2777782 [Escherichia coli]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T94260 NZ_CP026351:2777453-2777782 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT94260 WP_000072690.1 NZ_CP026351:2777115-2777432 [Escherichia coli]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT94260 NZ_CP026351:2777115-2777432 [Escherichia coli]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References