Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 2143146..2143693 Replicon chromosome
Accession NZ_CP026275
Organism Klebsiella oxytoca strain KONIH5

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0H3H5T3
Locus tag C2U46_RS10495 Protein ID WP_014227299.1
Coordinates 2143385..2143693 (+) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0H3H0P7
Locus tag C2U46_RS10490 Protein ID WP_014227298.1
Coordinates 2143146..2143382 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U46_RS10475 2139353..2140519 + 1167 WP_014227296.1 multidrug effflux MFS transporter -
C2U46_RS10480 2140680..2141582 + 903 WP_009654080.1 N-acetylmuramic acid 6-phosphate etherase -
C2U46_RS10485 2141607..2143040 + 1434 WP_014227297.1 PTS N-acetylmuramic acid transporter subunit IIBC -
C2U46_RS10490 2143146..2143382 + 237 WP_014227298.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
C2U46_RS10495 2143385..2143693 + 309 WP_014227299.1 CcdB family protein Toxin
C2U46_RS10500 2143844..2144746 + 903 WP_014227300.1 EamA family transporter -
C2U46_RS10505 2144750..2145007 - 258 Protein_1997 hypothetical protein -
C2U46_RS10510 2145007..2145984 - 978 WP_032695133.1 Rpn family recombination-promoting nuclease/putative transposase -
C2U46_RS10515 2146194..2146487 + 294 WP_014227302.1 hypothetical protein -
C2U46_RS10520 2146511..2147452 - 942 WP_014227303.1 LacI family transcriptional regulator -
C2U46_RS10525 2147747..2148058 + 312 WP_014227304.1 PTS sugar transporter subunit IIB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11725.57 Da        Isoelectric Point: 7.2729

>T93784 WP_014227299.1 NZ_CP026275:2143385-2143693 [Klebsiella oxytoca]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGEE
VCNLNHQREVVKASMDFLFDGI

Download         Length: 309 bp

>T93784 NZ_CP026275:2143385-2143693 [Klebsiella oxytoca]
ATGCAGTATTACGTTTATAAGAATACAGGTCGAATTGCTGCGTATCCTTACCTCCTTGATGTACAGAGTGACATTATCGG
CAAGCGCAATACTCGGGTCGTGATTCCACTGTTTCCACTGAAAAACTATAAAGGTCCACGCGCCGATCGTTTAACACCGC
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACATCGTGTTCTGGGTGAAGAA
GTTTGTAATCTGAATCACCAGCGCGAAGTCGTTAAAGCTTCAATGGATTTTCTTTTCGACGGGATCTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8783.92 Da        Isoelectric Point: 5.7928

>AT93784 WP_014227298.1 NZ_CP026275:2143146-2143382 [Klebsiella oxytoca]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT93784 NZ_CP026275:2143146-2143382 [Klebsiella oxytoca]
ATGCCAGCCGCAAAACGCAATAAGCAGAACGTCAGCGTGACACTGGACCCAGGCCTGGTCAAACAGGCTCGTGAGGTCGG
CATCAATCTGTCTCTAACGTTGACTAACGCTCTAAAAAGCGAAATCCACAGCCTCGAAATAGCCCGCTGGCAAAAAGAAA
ATGCCCAGGCAATCGACTACCTCAATAATTTAACTGAGGAAAATGGACTTCTCTCTGATGAATACAGGACGTTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H5T3

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H0P7

References