Detailed information of TA system TA093684

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 81556..81825
• Replicon: plasmid pNDM-d2e9
• Accession: NZ_CP026201
• Organism: Escherichia coli strain ECONIH6

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: C2U45_RS26135
• Protein ID: WP_001312861.1
• Coordinates: 81667..81825 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	81556..81621 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U45_RS26110	77365..77862	+	498	WP_095719660.1	single-stranded DNA-binding protein	-
C2U45_RS26115	77925..78158	+	234	WP_000005990.1	DUF905 family protein	-
C2U45_RS26120	78224..80182	+	1959	WP_001825193.1	ParB/RepB/Spo0J family partition protein	-
C2U45_RS26125	80237..80671	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
C2U45_RS26130	80668..81387	+	720	WP_013362833.1	plasmid SOS inhibition protein A	-
C2U45_RS26565	81399..81587	-	189	WP_001299721.1	hypothetical protein	-
-	81399..81623	+	225	NuclAT_0	-	-
-	81399..81623	+	225	NuclAT_0	-	-
-	81399..81623	+	225	NuclAT_0	-	-
-	81399..81623	+	225	NuclAT_0	-	-
-	81556..81621	+	66	-	-	Antitoxin
C2U45_RS26135	81667..81825	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
C2U45_RS26140	82220..83887	-	1668	WP_012372796.1	group II intron reverse transcriptase/maturase	-
C2U45_RS26155	85090..85377	+	288	WP_000107544.1	hypothetical protein	-
C2U45_RS26160	85495..86316	+	822	WP_001234469.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / mph(A) / blaTEM-1B / rmtB / blaNDM-5 / sul1 / qacE / aadA2 / dfrA12	-	1..100989	100989

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T93684 WP_001312861.1 NZ_CP026201:81667-81825 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T93684 NZ_CP026201:81667-81825 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT93684 NZ_CP026201:81556-81621 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin