Detailed information of TA system TA093671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 5238811..5239408
• Replicon: chromosome
• Accession: NZ_CP026178
• Organism: Klebsiella pneumoniae strain KPNIH49

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C2U49_RS26855
• Protein ID: WP_103215750.1
• Coordinates: 5238811..5239128 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: C2U49_RS26860
• Protein ID: WP_004142561.1
• Coordinates: 5239121..5239408 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U49_RS26835	5234511..5235938	-	1428	WP_040236578.1	MFS transporter	-
C2U49_RS26840	5236047..5236913	+	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
C2U49_RS26850	5237583..5238626	+	1044	WP_040236579.1	DUF2157 domain-containing protein	-
C2U49_RS26855	5238811..5239128	+	318	WP_103215750.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C2U49_RS26860	5239121..5239408	+	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
C2U49_RS26865	5239673..5240326	-	654	WP_004176973.1	oxygen-insensitive NAD(P)H nitroreductase	-
C2U49_RS26870	5240446..5240814	-	369	WP_040236580.1	MmcQ/YjbR family DNA-binding protein	-
C2U49_RS26875	5240814..5241395	-	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
C2U49_RS26880	5241575..5242690	+	1116	WP_012737592.1	MBL fold metallo-hydrolase	-
C2U49_RS26885	5242721..5243062	+	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
C2U49_RS26890	5243080..5243328	-	249	WP_002893037.1	DUF1158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12120.43 Da
• Isoelectric Point: 11.2767

>T93671 WP_103215750.1 NZ_CP026178:5238811-5239128 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSPEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T93671 NZ_CP026178:5238811-5239128 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCACCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT93671 WP_004142561.1 NZ_CP026178:5239121-5239408 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT93671 NZ_CP026178:5239121-5239408 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGGGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1