Detailed information of TA system TA093452

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 19281..19707
• Replicon: plasmid pF127_1
• Accession: NZ_CP026141
• Organism: Klebsiella pneumoniae strain F127

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: C2L73_RS30600
• Protein ID: WP_001312861.1
• Coordinates: 19281..19439 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	19483..19707 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2L73_RS27910	14336..14563	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
C2L73_RS27915	14657..15343	-	687	WP_015059009.1	PAS domain-containing protein	-
C2L73_RS27920	15534..15917	-	384	WP_000124981.1	relaxosome protein TraM	-
C2L73_RS27925	16194..16841	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
C2L73_RS27930	17138..17959	-	822	WP_001234445.1	DUF945 domain-containing protein	-
C2L73_RS27935	18070..18366	-	297	WP_001272251.1	hypothetical protein	-
C2L73_RS30920	18666..19043	+	378	Protein_28	hypothetical protein	-
C2L73_RS30600	19281..19439	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	19483..19707	-	225	NuclAT_0	-	Antitoxin
-	19483..19707	-	225	NuclAT_0	-	Antitoxin
-	19483..19707	-	225	NuclAT_0	-	Antitoxin
-	19483..19707	-	225	NuclAT_0	-	Antitoxin
C2L73_RS27960	19719..20438	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
C2L73_RS27965	20435..20869	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
C2L73_RS27970	20938..22961	-	2024	Protein_32	ParB/RepB/Spo0J family partition protein	-
C2L73_RS27975	23022..23255	-	234	WP_000006003.1	DUF905 family protein	-
C2L73_RS27980	23313..23840	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	rmtB / blaTEM-1B / blaCTX-M-65 / catA2 / blaKPC-2 / blaSHV-12	-	1..164501	164501

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T93452 WP_001312861.1 NZ_CP026141:c19439-19281 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T93452 NZ_CP026141:c19439-19281 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT93452 NZ_CP026141:c19707-19483 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin