Detailed information of TA system TA093359

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 16788..17439
• Replicon: plasmid pABA-2f10
• Accession: NZ_CP026129
• Organism: Acinetobacter baumannii strain ABNIH28

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0E2G754
• Locus tag: C2U32_RS19580
• Protein ID: WP_000269901.1
• Coordinates: 16788..17144 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C2U32_RS19585
• Protein ID: WP_001140619.1
• Coordinates: 17137..17439 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U32_RS20445	11951..12052	+	102	Protein_13	DNA-binding protein	-
C2U32_RS20245	12202..12426	+	225	WP_131202340.1	hypothetical protein	-
C2U32_RS20450	12460..12821	+	362	Protein_15	plasmid replication DNA-binding protein	-
C2U32_RS19560	13021..14346	-	1326	WP_001280604.1	ISNCY family transposase	-
C2U32_RS19570	14520..15032	-	513	WP_001089571.1	SMI1/KNR4 family protein	-
C2U32_RS19575	15711..16229	+	519	WP_000447197.1	sel1 repeat family protein	-
C2U32_RS19580	16788..17144	+	357	WP_000269901.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C2U32_RS19585	17137..17439	+	303	WP_001140619.1	XRE family transcriptional regulator	Antitoxin
C2U32_RS19590	17432..17617	+	186	WP_103270968.1	hypothetical protein	-
C2U32_RS19600	19061..19288	-	228	WP_000286812.1	hypothetical protein	-
C2U32_RS19610	20047..20592	-	546	Protein_24	IS5-like element ISAba13 family transposase	-
C2U32_RS19615	20520..20909	-	390	WP_002072923.1	AAA family ATPase	-
C2U32_RS19620	21097..21417	+	321	WP_000088946.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
C2U32_RS19625	21694..21882	+	189	WP_000033474.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C2U32_RS19630	21869..22156	+	288	WP_001258992.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..130044	130044
	-	inside	IScluster/Tn	-	-	13021..20472	7451

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13487.44 Da
• Isoelectric Point: 6.4631

>T93359 WP_000269901.1 NZ_CP026129:16788-17144 [Acinetobacter baumannii]
MWTVITTDLFNEWLAQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYEIHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T93359 NZ_CP026129:16788-17144 [Acinetobacter baumannii]
ATGTGGACAGTCATTACAACGGATCTGTTTAACGAATGGCTTGCACAGCAAGATGAATCTACACAAGAGAAAGTACTCGC
AGCGTTGGTCGTTCTACAGCAACAGGGACCAAGTTTAGGTCGTCCTTTAGTAGATACCGTCTATGACTCCAAATTTACCA
ATATGAAAGAGCTACGTGTTCAGCATCGAGGTAAACCACTGAGAGCCTTTTTCGCGTTTGATCCTTTACGACAAGCTATT
GTGCTGTGTATCGGTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGAGATGTTAGATATTGCCGATCAGCAATACGAAAT
TCATCTTTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11206.01 Da
• Isoelectric Point: 6.7282

>AT93359 WP_001140619.1 NZ_CP026129:17137-17439 [Acinetobacter baumannii]
MAKTLQELLANRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT93359 NZ_CP026129:17137-17439 [Acinetobacter baumannii]
ATGGCTAAGACCCTACAAGAATTATTGGCTAATCGCTCTCCAGAGAGTCAAGCTCGTATTCAACAAATGGCGGATGAATT
ATTGTTGGAAACTCAGCTTCACCTTATTCGTGAAGAGTTAGAGATTTCTCAAAAAGAACTGGCTGCAACCCTTGGAATCA
AACAGCCTTCATTGTCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTCGAAGCAATG
GGCGGAAAACTTCGTATAGATGTAGAGCTTCCAACAGGAAAACACATAGGATTCAATGTCTAA

Structures

Toxin

• AlphaFold DB: A0A0E2G754