Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 68781..69556 Replicon plasmid unnamed
Accession NZ_CP026099
Organism Shigella flexneri strain FDAARGOS_74

Toxin (Protein)


Gene name tacT Uniprot ID A0A896Z130
Locus tag MC63_RS23960 Protein ID WP_025759928.1
Coordinates 69074..69556 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q7BEG8
Locus tag MC63_RS23955 Protein ID WP_010921625.1
Coordinates 68781..69083 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MC63_RS23920 (MC63_025050) 63808..65262 + 1455 WP_000701110.1 type 3 secretion system effector OspC2 -
MC63_RS25075 65411..65539 - 129 Protein_76 IS5/IS1182 family transposase -
MC63_RS23935 (MC63_025060) 65711..66919 - 1209 WP_047564012.1 IS91 family transposase -
MC63_RS23940 (MC63_025065) 66900..67188 - 289 Protein_78 hypothetical protein -
MC63_RS23955 (MC63_025080) 68781..69083 + 303 WP_010921625.1 DUF1778 domain-containing protein Antitoxin
MC63_RS23960 (MC63_025085) 69074..69556 + 483 WP_025759928.1 GNAT family N-acetyltransferase Toxin
MC63_RS23965 (MC63_025090) 69648..70171 + 524 Protein_82 transposase -
MC63_RS23970 (MC63_025095) 70214..70552 - 339 Protein_83 integrase core domain-containing protein -
MC63_RS23975 (MC63_025105) 70626..71794 + 1169 WP_094099555.1 IS3-like element IS911 family transposase -
MC63_RS23980 (MC63_025110) 71818..72423 - 606 Protein_85 type III secretion system effector cysteine methyltransferase OspZ -
MC63_RS23985 (MC63_025115) 72481..73178 + 698 Protein_86 IS1 family transposase -
MC63_RS23990 (MC63_025120) 73195..73287 - 93 Protein_87 T3SS effector protein NleE -
MC63_RS23995 (MC63_025125) 73343..73495 + 153 Protein_88 integrase core domain-containing protein -
MC63_RS25395 (MC63_025130) 73777..73914 - 138 WP_011378983.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospI / ipaH9.8 / ospG / ospC2 / icsP/sopA / ospB / ospC3 / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ospE1 / ipaH1.4 / ipaH7.8 / ipaH4.5 / ospC1 / ospD3/senA / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG 1..229413 229413
- inside IScluster/Tn - ospC2 / icsP/sopA / ospB / ospC3 / ospC4 / ospC2 58872..82697 23825


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(29-149)

Antitoxin

Vibrio phage ICP1, Orf50

(19-94)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17749.54 Da        Isoelectric Point: 7.4014

>T93311 WP_025759928.1 NZ_CP026099:69074-69556 [Shigella flexneri]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDNARAFYERFGFVQSVVAPNTLFYKV

Download         Length: 483 bp

>T93311 NZ_CP026099:69074-69556 [Shigella flexneri]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTATTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATAACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11689.28 Da        Isoelectric Point: 4.9167

>AT93311 WP_010921625.1 NZ_CP026099:68781-69083 [Shigella flexneri]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Download         Length: 303 bp

>AT93311 NZ_CP026099:68781-69083 [Shigella flexneri]
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A896Z130

Antitoxin

Source ID Structure
AlphaFold DB Q7BEG8

References