Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 20662..21187 Replicon plasmid unnamed1
Accession NZ_CP026048
Organism Raoultella planticola strain FDAARGOS_64

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag MC50_RS26525 Protein ID WP_013023785.1
Coordinates 20662..20967 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag MC50_RS26530 Protein ID WP_001568025.1
Coordinates 20969..21187 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MC50_RS26495 16349..16975 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
MC50_RS28740 16972..17274 + 303 WP_071571079.1 hypothetical protein -
MC50_RS26505 17738..18532 - 795 WP_004197635.1 site-specific integrase -
MC50_RS26510 18730..19746 - 1017 WP_017899884.1 hypothetical protein -
MC50_RS26515 19757..20071 - 315 WP_053389906.1 hypothetical protein -
MC50_RS26520 20098..20493 - 396 WP_017899885.1 hypothetical protein -
MC50_RS26525 20662..20967 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
MC50_RS26530 20969..21187 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
MC50_RS26540 21357..21557 - 201 Protein_31 hypothetical protein -
MC50_RS26545 21742..22431 + 690 WP_004193995.1 hypothetical protein -
MC50_RS26550 22463..23152 - 690 WP_020315560.1 RES family NAD+ phosphorylase -
MC50_RS26555 23589..23837 + 249 WP_000272716.1 hypothetical protein -
MC50_RS26560 23834..24406 + 573 WP_020805590.1 hypothetical protein -
MC50_RS26565 24437..24931 + 495 WP_020805591.1 hypothetical protein -
MC50_RS26570 24975..25343 + 369 WP_004197807.1 hypothetical protein -
MC50_RS26575 25377..25580 + 204 WP_004197808.1 hemolysin expression modulator Hha -
MC50_RS26580 25594..25797 + 204 WP_004197809.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..127313 127313


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T92934 WP_013023785.1 NZ_CP026048:c20967-20662 [Raoultella planticola]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T92934 NZ_CP026048:c20967-20662 [Raoultella planticola]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT92934 WP_001568025.1 NZ_CP026048:c21187-20969 [Raoultella planticola]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT92934 NZ_CP026048:c21187-20969 [Raoultella planticola]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References