Detailed information of TA system TA092925

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4451750..4453353
• Replicon: chromosome
• Accession: NZ_CP026047
• Organism: Raoultella planticola strain FDAARGOS_64

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A8G2A023
• Locus tag: MC50_RS21330
• Protein ID: WP_032687736.1
• Coordinates: 4452022..4453353 (+)
• Length: 444 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2X2EM10
• Locus tag: MC50_RS21325
• Protein ID: WP_032696205.1
• Coordinates: 4451750..4452022 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MC50_RS21300	4447368..4448609	+	1242	WP_032699861.1	sensor domain-containing diguanylate cyclase	-
MC50_RS21305	4448628..4449260	+	633	WP_032699859.1	class I SAM-dependent methyltransferase	-
MC50_RS21310	4449476..4450429	+	954	WP_032687742.1	hypothetical protein	-
MC50_RS21315	4450561..4450986	+	426	WP_032687741.1	hypothetical protein	-
MC50_RS21320	4450992..4451546	+	555	WP_046852510.1	RNA polymerase sigma factor	-
MC50_RS21325	4451750..4452022	+	273	WP_032696205.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
MC50_RS21330	4452022..4453353	+	1332	WP_032687736.1	type II toxin-antitoxin system HipA family toxin	Toxin
MC50_RS21335	4453286..4454692	-	1407	WP_032699891.1	glycoside hydrolase family 10 protein	-
MC50_RS21340	4455405..4456463	+	1059	WP_162180097.1	branched-chain amino acid ABC transporter substrate-binding protein	-
MC50_RS21345	4456563..4457477	+	915	WP_015584333.1	branched-chain amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 444 a.a.
• Molecular weight: 49482.82 Da
• Isoelectric Point: 6.6648

>T92925 WP_032687736.1 NZ_CP026047:4452022-4453353 [Raoultella planticola]
MATLVTWMNNERVGELIKQANGAHLFRYEEAWMRNPRARPLSLSLPLQYGNITDDAVFNYFDNLLPDSPRVRDRIVKRYQ
ARSKQPFDLLAEIGRDSVGAVTLLPPGENATRRALSWEALDSHALDALLTAYQSDIPLGMVNEHDDFRISVAGAQEKTAL
LKIGEQWCIPTGTTPTTHIIKLPIGEIKQPDAVLDLSESVDNEYLCLALARALGFAVPRASIIQTASIRALAVERFDRRW
AQEKTVLLRLPQEDLCQAFGIPSSIKYESDGGPGIRAIMAFLVGSSEALEDRYNFMKFMVFQWLIGATDGHAKNFSIFLQ
PGGSYRLTPFYDIISAFPLLGGKGLHLSDLRLSMSLKASKGRKTEIQTLYPRHFLATAKEVGFAEEQMLEILRYFVDNVP
HAVAAVKETLPHDFSPPVYEAITDRLLWVHSRLQNAMAALPSG

Nucleotide

• Length: 1332 bp

>T92925 NZ_CP026047:4452022-4453353 [Raoultella planticola]
ATGGCTACTCTCGTGACATGGATGAACAATGAGCGAGTCGGCGAACTTATCAAGCAGGCGAACGGCGCCCATCTCTTTCG
CTATGAAGAGGCGTGGATGCGCAATCCTCGCGCCAGGCCGCTCTCTCTGTCGCTCCCGCTGCAGTACGGTAACATCACCG
ACGATGCGGTGTTTAACTATTTTGACAACCTGCTGCCGGACAGCCCGCGGGTGCGGGACCGCATTGTTAAGCGCTATCAG
GCGCGTTCAAAGCAGCCTTTTGATCTGCTGGCCGAGATCGGACGCGACAGCGTCGGGGCGGTCACGTTACTCCCGCCAGG
GGAAAACGCAACCCGGAGAGCGTTGAGTTGGGAGGCGCTGGATAGCCACGCCCTGGATGCGCTGCTGACGGCCTACCAGT
CGGATATACCGCTCGGGATGGTCAACGAACACGATGATTTCAGGATCTCGGTGGCCGGGGCCCAGGAGAAAACCGCGCTG
CTGAAAATCGGCGAGCAGTGGTGTATACCGACGGGGACCACGCCGACCACCCATATCATTAAACTGCCTATCGGCGAGAT
CAAACAGCCGGATGCGGTGCTGGATCTCAGCGAAAGCGTAGATAACGAATATTTGTGTCTGGCGCTGGCCCGGGCGCTGG
GTTTTGCCGTTCCGCGCGCCAGCATTATCCAGACCGCCAGCATCAGGGCGCTGGCGGTTGAGCGTTTCGACCGTCGTTGG
GCGCAGGAGAAGACGGTGCTGCTGCGTTTACCGCAGGAAGATCTGTGTCAGGCCTTCGGTATTCCGTCATCGATTAAGTA
TGAGTCCGACGGCGGGCCGGGCATTCGGGCGATTATGGCTTTTCTGGTTGGATCCAGCGAGGCGCTCGAGGATCGCTATA
ACTTTATGAAGTTTATGGTGTTCCAGTGGCTGATTGGCGCAACCGATGGCCACGCGAAAAACTTCTCGATCTTCCTCCAG
CCGGGCGGCAGCTATCGCTTAACCCCATTCTACGACATTATCTCCGCGTTTCCGCTGCTTGGCGGGAAGGGGCTGCACCT
GAGCGATCTCCGGCTGTCAATGAGCCTGAAGGCCAGTAAAGGGCGCAAAACGGAGATCCAGACGCTCTATCCGCGTCATT
TCCTCGCCACGGCGAAAGAGGTGGGGTTTGCCGAGGAGCAGATGCTGGAGATTCTGCGCTATTTTGTCGATAACGTTCCC
CACGCGGTTGCTGCGGTAAAGGAAACTTTACCGCATGACTTTTCACCACCGGTGTATGAGGCGATCACCGATCGCTTACT
GTGGGTCCATTCACGCTTGCAGAACGCAATGGCGGCGTTACCGTCGGGCTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10168.43 Da
• Isoelectric Point: 4.9491

>AT92925 WP_032696205.1 NZ_CP026047:4451750-4452022 [Raoultella planticola]
MSPSPGIYSPVQLANYLKLVRQKNQWTQSDLAQRMGIKQATVSNFENHPEKTTLTTVFKILQSLELSMAICEKNAPDTND
SSDNPQDLDW

Nucleotide

• Length: 273 bp

>AT92925 NZ_CP026047:4451750-4452022 [Raoultella planticola]
ATGAGCCCTTCACCAGGAATATACAGCCCGGTTCAGCTGGCAAACTATCTCAAGCTGGTGCGCCAGAAAAACCAGTGGAC
GCAGAGCGATCTGGCTCAGCGTATGGGGATTAAACAGGCTACCGTCTCTAACTTTGAAAATCATCCCGAGAAAACGACGT
TAACCACGGTGTTTAAAATTTTACAGTCACTGGAACTGTCGATGGCGATTTGCGAAAAAAATGCGCCGGATACCAACGAT
AGCAGTGATAACCCACAGGATCTGGACTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2X2EM10