Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2990367..2990986 Replicon chromosome
Accession NZ_CP026047
Organism Raoultella planticola strain FDAARGOS_64

Toxin (Protein)


Gene name Hha Uniprot ID A0A514EV16
Locus tag MC50_RS14500 Protein ID WP_004858783.1
Coordinates 2990367..2990585 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A514EV88
Locus tag MC50_RS14505 Protein ID WP_004858785.1
Coordinates 2990612..2990986 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MC50_RS14470 2986441..2986701 + 261 WP_032689208.1 type B 50S ribosomal protein L31 -
MC50_RS14475 2986704..2986844 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
MC50_RS14480 2986841..2987551 - 711 WP_047664237.1 GNAT family N-acetyltransferase -
MC50_RS14485 2987653..2989104 + 1452 WP_047664238.1 PLP-dependent aminotransferase family protein -
MC50_RS14490 2989079..2989549 - 471 WP_004858780.1 YlaC family protein -
MC50_RS14495 2989649..2990215 - 567 WP_032689205.1 maltose O-acetyltransferase -
MC50_RS14500 2990367..2990585 - 219 WP_004858783.1 hemolysin expression modulator Hha Toxin
MC50_RS14505 2990612..2990986 - 375 WP_004858785.1 Hha toxicity modulator TomB Antitoxin
MC50_RS14510 2991514..2994660 - 3147 WP_032689204.1 multidrug efflux RND transporter permease subunit -
MC50_RS14515 2994683..2995876 - 1194 WP_032689203.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8570.90 Da        Isoelectric Point: 7.9907

>T92924 WP_004858783.1 NZ_CP026047:c2990585-2990367 [Raoultella planticola]
MSGEPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T92924 NZ_CP026047:c2990585-2990367 [Raoultella planticola]
ATGTCTGGTGAACCATTAACCAAAACAGACTATTTGATGCGCTTACGTCGCTGTCAGACAATTGATACGCTGGAACGCGT
AATTGAAAAAAACAAATATGAACTTTCAGATAACGAACTGGCTGTATTTTACTCAGCGGCCGATCACCGTCTTGCTGAAT
TGACCATGAATAAACTCTACGACAAAATCCCGACATCGGTGTGGAAGTTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14411.19 Da        Isoelectric Point: 4.8989

>AT92924 WP_004858785.1 NZ_CP026047:c2990986-2990612 [Raoultella planticola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYAEDNKLIAQLDEYL
DDTFMLFSSYGINTADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT92924 NZ_CP026047:c2990986-2990612 [Raoultella planticola]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCGCAGCTTCGATTTCTTTGCGAAACCCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGTAACCACGGGTGGGTGAATGACCCGACGTCGGCTATCAATCTTCAACTTAATGAATTGATCGAAC
ACATAGCCACGTTCGCGCTTAATTATAAAATTAAGTATGCTGAGGATAATAAACTGATCGCTCAGCTGGACGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGCATTAACACGGCCGATCTACAGAAATGGCGCAAATCGGGAAATCGGTT
ATTCCGATGTTTTGTCAACGCCAGCCGGGAAAATCCGGCAAGTCTTTCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EV16

Antitoxin

Source ID Structure
AlphaFold DB A0A514EV88

References